Division of Constitutive and Regenerative Sciences, School of Dentistry, University of California, Los Angeles, CA, USA.
Department of Diagnostic and Biological Sciences, School of Dentistry, Universit of Minnesota, Minneapolis, MN, USA.
Microb Ecol. 2019 Aug;78(2):336-347. doi: 10.1007/s00248-018-1291-0. Epub 2018 Nov 24.
Staphylococcus aureus, an opportunistic pathogen member of the nasal and skin microbiota, can also be found in human oral samples and has been linked to infectious diseases of the oral cavity. As the nasal and oral cavities are anatomically connected, it is currently unclear whether S. aureus can colonize the oral cavity and become part of the oral microbiota, or if its presence in the oral cavity is simply transient. To start addressing this question, we assessed S. aureus ability to directly bind selected members of the oral microbiota as well as its ability to integrate into a human-derived complex oral microbial community in vitro. Our data show that S. aureus forms aggregates with Fusobacterium nucleatum and Porphyromonas gingivalis and that it can incorporate into the human-derived in vitro oral community. Further analysis of the F. nucleatum-S. aureus interaction revealed that the outer-membrane adhesin RadD is partially involved in aggregate formation and that the RadD-mediated interaction leads to an increase in expression of the staphylococcal global regulator gene sarA. Our findings lend support to the notion that S. aureus can become part of the complex microbiota of the human mouth, which could serve as a reservoir for S. aureus. Furthermore, direct interaction with key members of the oral microbiota could affect S. aureus pathogenicity contributing to the development of several S. aureus associated oral infections.
金黄色葡萄球菌是一种机会性病原体，属于鼻腔和皮肤微生物群的成员，也可以在人类口腔样本中发现，并与口腔传染病有关。由于鼻腔和口腔在解剖上是相连的，目前尚不清楚金黄色葡萄球菌是否可以定植于口腔并成为口腔微生物群的一部分，或者其在口腔中的存在是否只是短暂的。为了开始解决这个问题，我们评估了金黄色葡萄球菌直接结合口腔微生物群选定成员的能力，以及其在体外整合到人类来源的复杂口腔微生物群落中的能力。我们的数据表明，金黄色葡萄球菌与核梭杆菌和牙龈卟啉单胞菌形成聚集物，并且可以整合到人类来源的体外口腔群落中。对 F. nucleatum-S. aureus 相互作用的进一步分析表明，外膜粘附素 RadD 部分参与聚集物的形成，并且 RadD 介导的相互作用导致葡萄球菌全局调节基因 sarA 的表达增加。我们的研究结果支持这样一种观点，即金黄色葡萄球菌可以成为人类口腔复杂微生物群的一部分，这可能成为金黄色葡萄球菌的储存库。此外，与口腔微生物群的关键成员的直接相互作用可能会影响金黄色葡萄球菌的致病性，导致几种与金黄色葡萄球菌相关的口腔感染的发生。
