Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing, 100871, China.
Institute for Advanced Study, Shenzhen University, Nanshan District, Shenzhen, Guangdong, 518060, China.
Mol Nutr Food Res. 2019 Feb;63(3):e1800808. doi: 10.1002/mnfr.201800808. Epub 2018 Dec 5.
The aim of the present study is to investigate the efficacy of Nitzschia laevis extract (NLE) in preventing obesity in mice fed with a high-fat diet (HFD), and the potential underlying mechanisms focusing on modulation of the gut microbiota profile.
Physiological, histological, and biochemical parameters and gut microbiota compositions are compared among four experimental groups fed respectively with the following diets for 8 weeks: Normal chow diet, HFD, HFD + low concentration of NLE, and HFD + high concentration of NLE. The results demonstrate that NLE supplementation significantly reduces body weight gain and effectively prevents lipid accumulation in the white adipose tissue and liver of the mice. Mechanistic analysis reveals that NLE promotes the expression of uncoupling protein 1 and peroxisome proliferators-activated receptor-γ coactivator-1 mRNA in brown adipose tissue. Furthermore, NLE protects the gut epithelium and positively reshapes the gut microbiota composition against the damaging effect of HFD.
NLE supplementation demonstrates a protective effect against HFD-induced obesity in mice, which is associated with reshaping the profile of gut microbiota. To the best of our knowledge, this has been the first report on the potential of microalgal extract to prevent obesity by modulating gut microbiota.
本研究旨在探讨菱形藻提取物（NLE）在预防高脂肪饮食（HFD）喂养小鼠肥胖中的功效，以及通过调节肠道微生物群谱来预防肥胖的潜在机制。
在 8 周的时间里，分别用以下饮食喂养四组实验动物：正常饲料、HFD、HFD+低浓度 NLE 和 HFD+高浓度 NLE，比较其生理、组织学和生化参数以及肠道微生物群组成。结果表明，NLE 补充剂可显著减少体重增加，并有效防止白色脂肪组织和肝脏中的脂肪堆积。机制分析表明，NLE 可促进棕色脂肪组织解偶联蛋白 1 和过氧化物酶体增殖物激活受体-γ 共激活因子 1 mRNA 的表达。此外，NLE 可保护肠道上皮细胞，并对 HFD 的破坏性影响积极重塑肠道微生物群组成。
NLE 补充剂对 HFD 诱导的肥胖具有保护作用，这与重塑肠道微生物群组成有关。据我们所知，这是首次报道微藻提取物通过调节肠道微生物群来预防肥胖的潜力。
