Li Lingfei, Ma Li, Wen Yanlong, Xie Jing, Yan Liang, Ji Aibing, Zeng Yin, Tian Yang, Sheng Jun
College of Food Science and Technology, Yunnan Agricultural University, Kunming, China.
Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, China.
Front Nutr. 2022 Apr 25;9:861588. doi: 10.3389/fnut.2022.861588. eCollection 2022.
is a commonly used plant with high nutritional and medicinal values. leaves are considered a new food resource in China. However, the biological activities of polysaccharides (MOP) in regulating gut microbiota and alleviating obesity remain obscure. In the present study, we prepared the MOP and evaluated its effects on obesity and gut microbiota in high-fat diet (HFD)-induced C57BL/6J mice. The experimental mice were supplemented with a normal chow diet (NCD group), a high-fat diet (HFD group), and HFD along with MOP at a different dose of 100, 200, and 400 mg/kg/d, respectively. Physiological, histological, biochemical parameters, genes related to lipid metabolism, and gut microbiota composition were compared among five experimental groups. The results showed that MOP supplementation effectively prevented weight gain and lipid accumulation induced by HFD, ameliorated blood lipid levels and insulin resistance, alleviated the secretion of pro-inflammatory cytokines, and regulated the expression of genes related to lipid metabolism and bile acid metabolism. In addition, MOP positively reshaped the gut microbiota composition, significantly increasing the abundance of , norank_f_Ruminococcaceae, and , while decreasing the relative abundance of , and , which are closely associated with obesity. These results demonstrated that MOP supplementation has a protective effect against HFD-induced obesity in mice, which was associated with reshaping the gut microbiota. To the best of our knowledge, this is the first report on the potential of MOP to prevent obesity and modulating gut microbiota, which suggests that MOP can be used as a potential prebiotic.
是一种常用的具有高营养和药用价值的植物。其叶子在中国被视为一种新的食物资源。然而，多糖（MOP）在调节肠道微生物群和缓解肥胖方面的生物活性仍不清楚。在本研究中，我们制备了MOP，并评估了其对高脂饮食（HFD）诱导的C57BL/6J小鼠肥胖和肠道微生物群的影响。实验小鼠分别补充正常饲料饮食（NCD组）、高脂饮食（HFD组）以及不同剂量（100、200和400 mg/kg/d）的MOP与高脂饮食。比较了五个实验组的生理、组织学、生化参数、与脂质代谢相关的基因以及肠道微生物群组成。结果表明，补充MOP有效预防了HFD诱导的体重增加和脂质积累，改善了血脂水平和胰岛素抵抗，减轻了促炎细胞因子的分泌，并调节了与脂质代谢和胆汁酸代谢相关的基因表达。此外，MOP积极重塑了肠道微生物群组成，显著增加了[未提及具体菌属1]、未分类_f_瘤胃球菌科和[未提及具体菌属2]的丰度，同时降低了与肥胖密切相关的[未提及具体菌属3]、[未提及具体菌属4]和[未提及具体菌属5]的相对丰度。这些结果表明，补充MOP对HFD诱导的小鼠肥胖具有保护作用，这与重塑肠道微生物群有关。据我们所知，这是关于MOP预防肥胖和调节肠道微生物群潜力的首次报道，这表明MOP可作为一种潜在的益生元。
