显微镜用动态非接触式三维样品旋转。

Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307, Dresden, Germany.

Max Planck Institute for the Physics of Complex Systems, Nöthnitzer Str. 38, 01187, Dresden, Germany.

Nat Commun. 2018 Nov 28;9(1):5025. doi: 10.1038/s41467-018-07504-3.

Precise sample orientation is crucial for microscopy but is often performed with macroscopic tools and low accuracy. In vivo imaging of growing and developing samples even requires dynamic adaptation of the sample orientation to continuously achieve optimal imaging. Here, we present a method for freely positioning a sample in 3D by introducing magnetic beads and applying a magnetic field. We demonstrate magnetic orientation of fixed mouse embryos and artemia, and live zebrafish embryos and larvae on an epi-fluorescence microscope and on a light-sheet system for optimal imaging.

精确的样本定位对于显微镜来说至关重要，但通常使用宏观工具且精度不高。在对生长和发育中的样本进行活体成像时，甚至需要动态调整样本的方向，以持续获得最佳的成像效果。在这里，我们通过引入磁性珠并施加磁场的方法，展示了一种能够在 3D 中自由定位样本的方法。我们在荧光显微镜和光片系统上演示了固定的小鼠胚胎和卤虫、活体斑马鱼胚胎和幼虫的磁性定位，以实现最佳成像。