Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Zhejiang, Hangzhou, China.
Department of Statistics, The University of Georgia, Athens, GA, USA.
Biofactors. 2018 Nov;44(6):577-587. doi: 10.1002/biof.1454. Epub 2018 Nov 29.
Harsh climate induces physiological stress thus compromising organismal survival. Our previous studies demonstrated that curcumin (CUR) supplementation increased survival of turtle under heat stress (HS). Here, we span this work to investigate the survival and lifespan of HS Drosophila fed a diet supplemented with CUR. For this purpose, female and male flies were fed basal diet (N) and CUR diet (0.2 mg/g), and exposed to three conditions: 25°C and 29°C continuously, and 34 °C for 2 h at days 1, 4, and 7, then kept at 25 °C. Lifespan analysis showed that, compared to N-25 °C flies, the mean lifespans of N-29 °C and N-34 °C flies were decreased significantly by 8.5-15.7% in males, and 3.7-7.9% in females. Conversely, in the CUR-supplemented diet, mean lifespans of C-29 °C and C-34 °C flies were significantly extended by 8.7-16.4% in males, and by 8.9-12.8% in females, compared to that of temperature-matched flies fed basal diets. The MDA levels of C-34 °C flies were significantly lower than those of N-34 °C flies, indicating CUR reduced oxidative stress caused by HS. Furthermore, CUR palliated the increased oxidative stress caused by HS, by increasing the expression of SOD1, CAT, and PHGPx and decreasing the expression of Hsp70 and Hsp83. Our results indicated that CUR supplementation increases the survival rate of Drosophila by enhancing thermal tolerance. © 2018 BioFactors, 44(6):577-587, 2018.
恶劣的气候会导致生理压力，从而影响生物的生存。我们之前的研究表明，姜黄素（CUR）补充剂可以提高海龟在热应激（HS）下的存活率。在这里，我们将这项工作扩展到研究补充 CUR 饮食的 HS 果蝇的存活率和寿命。为此，雌性和雄性果蝇分别喂食基础饮食（N）和 CUR 饮食（0.2mg/g），并暴露于三种条件下：连续 25°C 和 29°C，以及第 1、4 和 7 天每天 34°C 持续 2 小时，然后保持在 25°C。寿命分析表明，与 N-25°C 组相比，N-29°C 和 N-34°C 组的雄性果蝇平均寿命分别缩短了 8.5-15.7%，雌性果蝇平均寿命分别缩短了 3.7-7.9%。相反，在补充 CUR 的饮食中，与温度匹配的喂食基础饮食的果蝇相比，C-29°C 和 C-34°C 组的雄性和雌性果蝇的平均寿命分别延长了 8.7-16.4%和 8.9-12.8%。C-34°C 组的 MDA 水平明显低于 N-34°C 组，表明 CUR 减轻了 HS 引起的氧化应激。此外，CUR 通过增加 SOD1、CAT 和 PHGPx 的表达和降低 Hsp70 和 Hsp83 的表达，缓解了 HS 引起的氧化应激增加。我们的研究结果表明，CUR 补充剂通过提高耐热性来提高果蝇的存活率。© 2018 BioFactors, 44(6):577-587, 2018.
