Aragón-Rojas Stephania, Quintanilla-Carvajal María Ximena, Hernández-Sánchez Humberto
Biosciences Doctoral Program, Faculty of Engineering, University of La Sabana, Common Campus Bridge, Km. 7 Bogota North Freeway, Chía, 140013 Cundinamarca, Colombia.
National School of Biological Sciences, National Polytechnic Institute, Av. Wilfrido Massieu esq. Cda. M. Stampa, UP Adolfo López Mateos, 07738 Ciudad de Mexico, Mexico.
Food Technol Biotechnol. 2018 Sep;56(3):381-397. doi: 10.17113/ftb.56.03.18.5285.
This study aims to evaluate the multifunctional role of whey culture medium during the spray drying microencapsulation of K73. Whey culture medium containing growing microorganisms served to hydrate different mixtures (gum arabic, maltodextrin and whey). We evaluated the use of these mixtures as carbon sources and their protective effects on simulated gastrointestinal conditions. The optimal mixture was spray-dried while varying the outlet temperature and atomizing pressure using a response surface design. These conditions served to evaluate microorganism survival, tolerance to gastrointestinal conditions , physicochemical properties, morphometric features and stability at 4, 25 and 37 °C. K73 replicated in the carrier material. Bacterial change cycles were (-1.97±0.16) log CFU/g after the drying process and (-0.61±0.08) and (-0.23±0.00) log CFU/g after exposure of the capsules to simulated gastric pH and bile salt content, respectively. The physicochemical properties and morphometric features were within the normal ranges for a powder product. The powder was stable at a storage temperature of 4 °C. The spray drying of the whey culture medium with growing microorganisms using the optimized drying conditions was successful. This study demonstrates the use of whey culture medium as a component of carrier material or as the carrier material itself, as well as its protective effects during drying, under simulated gastrointestinal conditions, and at varied storage temperatures.
本研究旨在评估乳清培养基在K73喷雾干燥微胶囊化过程中的多功能作用。含有生长中微生物的乳清培养基用于水化不同的混合物（阿拉伯胶、麦芽糊精和乳清）。我们评估了这些混合物作为碳源的用途及其在模拟胃肠道条件下的保护作用。使用响应面设计，在改变出口温度和雾化压力的同时对最佳混合物进行喷雾干燥。这些条件用于评估微生物的存活率、对胃肠道条件的耐受性、理化性质、形态特征以及在4、25和37°C下的稳定性。K73在载体材料中进行了复制。干燥过程后细菌数量变化周期为（-1.97±0.16）log CFU/g，胶囊分别暴露于模拟胃液pH值和胆汁盐含量后，细菌数量变化周期分别为（-0.61±0.08）log CFU/g和（-0.23±0.00）log CFU/g。理化性质和形态特征在粉末产品的正常范围内。该粉末在4°C储存温度下稳定。使用优化的干燥条件对含有生长中微生物的乳清培养基进行喷雾干燥取得了成功。本研究证明了乳清培养基可作为载体材料的一种成分或作为载体材料本身使用，以及其在干燥过程中、模拟胃肠道条件下和不同储存温度下的保护作用。
