Department of Gastroenterology, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.
Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing, China.
J Dig Dis. 2019 Jan;20(1):18-24. doi: 10.1111/1751-2980.12692. Epub 2018 Dec 27.
Changes in microbiota composition in the distal esophagus may be associated with the pathogenesis of gastroesophageal reflux disease. We aimed to compare the composition of distal esophageal microbiota between Chinese patients with reflux esophagitis (RE) and healthy volunteers using metagenomic high-throughput DNA sequencing and bioinformatic analyses.
Healthy volunteers (controls) and patients with reflux esophagitis (RE) were enrolled. Distal esophageal (2 cm above the gastroesophageal junction) biopsy specimens were obtained under endoscopy. Microbial DNA was extracted from the specimens, followed by 16S rDNA gene amplification and Illumina sequencing. Bioinformatic tools were applied to dissect the community structure.
No dramatic differences in microbiota were found in RE patients compared with the controls. At the phylum level, only Bacteroidetes differed between the groups, being less abundant in the RE group. The overall number and diversity of species tended to be lower in RE patients, but there were no significant differences between the groups. Three genera, Prevotella, Helicobacter, and Moraxella, were obviously depleted in RE patients, as revealed by linear discriminant analysis.
The composition of distal esophageal microbiota in Chinese patients with RE showed moderate changes compared with healthy controls. To what extent these changes are associated with the pathogenesis of RE needs further investigation.
远端食管微生物群落组成的改变可能与胃食管反流病的发病机制有关。本研究旨在通过宏基因组高通量 DNA 测序和生物信息学分析，比较中国反流性食管炎（RE）患者和健康志愿者远端食管微生物群落的组成。
纳入健康志愿者（对照组）和反流性食管炎（RE）患者。在内镜下采集远端食管（胃食管交界处上方 2cm）活检标本。从标本中提取微生物 DNA，然后进行 16S rDNA 基因扩增和 Illumina 测序。应用生物信息学工具分析群落结构。
与对照组相比，RE 患者的微生物群落无明显差异。在门水平上，仅拟杆菌门在两组之间存在差异，RE 组中含量较少。RE 患者的物种总数和多样性往往较低，但两组间无显著差异。线性判别分析显示，RE 患者中有 3 个属（普雷沃氏菌属、幽门螺杆菌属和莫拉菌属）明显减少。
与健康对照组相比，中国 RE 患者远端食管微生物群落组成有中度变化。这些变化在多大程度上与 RE 的发病机制有关，需要进一步研究。
