Wang Baoyong, Zhang Yu, Zhao Qiaofei, Yan Yifan, Yang Tian, Xia Yanli, Chen Hongwei
Department of Gastroenterology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, China.
Front Pharmacol. 2020 Jul 7;11:1000. doi: 10.3389/fphar.2020.01000. eCollection 2020.
Reflux Esophagitis (RE) is caused by a variety of factors including anatomical and functional alterations involved in the pathogenesis. Oral microbiota is influenced by many factors such as heredity, nutrition, environments and host conditions, but little is known about relationship between oral microbiota and RE. The aim of this study was to explore whether the oral microbiota is changed in patients with RE.
To clarify this correlation, fresh saliva samples from all subjects were collected and then oral microorganism diversity was analysed in 55 patients with RE and 51 controls hypervariable tag sequencing and analyzing the V3-V4 region of the 16S rDNA gene.
There was no difference found in oral microbial diversity between RE patients and healthy controls by Shannon diversity index (p=0.60) and Simpson diversity index (p= 0.38). The abundance of was lower, but was higher in patients with RE at the phylum level. At the genus level the abundances of , , , , , , and were increased, while , and were decreased in RE patients than the controls.
Our study suggested oral microbial dysbiosis in patients with RE, and identified bacterial species with potential biomarker significance. Further studies are required to understand role of oral microbial dysbiosis in the pathogenesis of RE.
反流性食管炎（RE）由多种因素引起，包括发病机制中涉及的解剖学和功能改变。口腔微生物群受遗传、营养、环境和宿主状况等多种因素影响，但关于口腔微生物群与RE之间的关系知之甚少。本研究旨在探讨RE患者的口腔微生物群是否发生变化。
为阐明这种相关性，收集了所有受试者的新鲜唾液样本，然后对55例RE患者和51例对照者的口腔微生物多样性进行了分析，采用高变标签测序并分析16S rDNA基因的V3-V4区域。
通过香农多样性指数（p=0.60）和辛普森多样性指数（p=0.38）发现，RE患者与健康对照者的口腔微生物多样性无差异。在门水平上，RE患者的丰度较低，但丰度较高。在属水平上，RE患者的、、、、、、和的丰度增加，而、和的丰度低于对照者。
我们的研究提示RE患者存在口腔微生物失调，并鉴定出具有潜在生物标志物意义的细菌种类。需要进一步研究以了解口腔微生物失调在RE发病机制中的作用。
