Souza G R, Oliveira-Junior R G, Diniz T C, Branco A, Lima-Saraiva S R G, Guimarães A L, Oliveira A P, Pacheco A G M, Silva M G, Moraes-Filho M O, Costa M P, Pessoa C Ó, Almeida J R G S
Núcleo de Estudos e Pesquisas de Plantas Medicinais, Universidade Federal do Vale do São Francisco, Petrolina, PE, Brazil.
Programa de Pós-graduação em Biotecnologia, Universidade Estadual de Feira de Santana, Feira de Santana, BA, Brazil.
Braz J Biol. 2018 May;78(2):248-254. doi: 10.1590/1519-6984.05316. Epub 2017 Aug 17.
This study was carried out to assess the antibacterial, cytotoxic and antioxidant activities of extracts of Morus nigra L. HPLC was used to determine the fingerprint chromatogram of the crude ethanolic extract (Mn-EtOH). The antibacterial effect was assessed through the method of microdilution. The cytotoxicity was tested against human tumour cell lines using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The total phenolic and flavonoid contents were also assessed through the Folin-Ciocalteu and aluminum chloride methods, respectively. Antioxidant activities of the extracts were evaluated by using 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging and β-carotene-linoleic acid bleaching methods. The presence of phenolic compounds in Mn-EtOH was confirmed using HPLC. The extracts showed activity against most microorganisms tested. The extracts did not show any expressive antiproliferative effect in the assessment of cytotoxicity. The most significant total phenolic content was 153.00 ± 11.34 mg of gallic acid equivalent/g to the ethyl acetate extract (AcOEt). The total flavonoid content was 292.50 ± 70.34 mg of catechin equivalent/g to the AcOEt extract, which presented the best antioxidant activity (IC50 50.40 ± 1.16 μg/mL) for DPPH scavenging. We can conclude that this species shows strong antibacterial and antioxidant activities, as well as weak cytotoxic effects.
本研究旨在评估黑桑提取物的抗菌、细胞毒性和抗氧化活性。采用高效液相色谱法测定粗乙醇提取物（Mn-EtOH）的指纹图谱。通过微量稀释法评估抗菌效果。使用3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四氮唑溴盐（MTT）法检测对人肿瘤细胞系的细胞毒性。还分别通过福林-西奥尔特法和氯化铝法评估总酚和黄酮含量。采用2,2-二苯基-1-苦基肼（DPPH）自由基清除法和β-胡萝卜素-亚油酸漂白法评估提取物的抗氧化活性。使用高效液相色谱法确认Mn-EtOH中酚类化合物的存在。提取物对大多数测试微生物显示出活性。在细胞毒性评估中，提取物未显示出任何明显的抗增殖作用。总酚含量最高的是乙酸乙酯提取物（AcOEt），为153.00±11.34mg没食子酸当量/g。乙酸乙酯提取物的总黄酮含量为292.50±70.34mg儿茶素当量/g，其对DPPH清除表现出最佳抗氧化活性（IC50为50.40±1.16μg/mL）。我们可以得出结论，该物种具有较强的抗菌和抗氧化活性，以及较弱的细胞毒性作用。
