Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Box 7624, Raleigh, NC 27695, United States.
Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Box 7624, Raleigh, NC 27695, United States.
Curr Opin Biotechnol. 2019 Apr;56:163-171. doi: 10.1016/j.copbio.2018.11.009. Epub 2018 Dec 11.
Our evolving understanding on the mechanisms underlying the health-promoting attributes of probiotic lactobacilli, together with an expanding genome editing toolbox have made this genus an ideal chassis for the development of living therapeutics. The rising adoption of CRISPR-based technologies for prokaryotic engineering has demonstrated precise, efficient and scalable genome editing and tunable transcriptional regulation that can be translated into next-generation development of probiotic lactobacilli with enhanced robustness and designer functionalities. Here, we discuss how these tools in conjunction with the naturally abundant and diverse native CRISPR-Cas systems can be harnessed for Lactobacillus cell surface engineering and the delivery of biotherapeutics.
我们对益生菌乳杆菌促进健康的特性的潜在机制的不断深入的理解，以及不断扩展的基因组编辑工具包，使这个属成为开发活体治疗药物的理想底盘。基于 CRISPR 的技术在原核工程中的应用越来越广泛，展示了精确、高效和可扩展的基因组编辑和可调转录调控，可转化为具有增强的稳健性和设计功能的益生菌乳杆菌的新一代开发。在这里，我们讨论了如何将这些工具与自然丰富多样的天然 CRISPR-Cas 系统结合起来，用于乳杆菌细胞表面工程和生物治疗药物的递送。
