Biological Materials Laboratory, Microbiology Division, CSIR-CLRI (Central Leather Reasearch Institute), Adyar, Chennai, India.
J Tissue Eng Regen Med. 2019 Feb;13(2):174-178. doi: 10.1002/term.2779. Epub 2018 Dec 25.
The present study emphasizes the role of engineered protein (gallic acid engineered gelatin [GEG]) on the closure of wound gaps of different shapes assessed under in vitro (fibroblast cell line) and in vivo (rat) experimental models. Circular, triangle, rectangle, and square are the shapes selected for the study. Intending engineered protein (GEG) augments the cell migration in rectangle and triangle shapes and reduces the gap space significantly compared with circular and square shapes. Similar observations were made with in vivo model study, and it was observed that the wound closure starts along the wound edges. In circular and square shapes, the cell movement follow a purse-string mechanism/the mixed pattern. Thus, the present study suggested that for faster wound healing, the cell migration along the wound edge may be found beneficial, and the external healing agent in the form of engineered protein hydrogel accelerate the healing accordingly.
本研究强调了工程蛋白（没食子酸工程明胶 [GEG]）在体外（成纤维细胞系）和体内（大鼠）实验模型评估的不同形状的伤口间隙闭合中的作用。选择圆形、三角形、矩形和正方形进行研究。目的工程蛋白（GEG）可促进矩形和三角形形状的细胞迁移，并与圆形和正方形形状相比显著减少间隙空间。在体内模型研究中也观察到了类似的观察结果，并且观察到伤口闭合沿着伤口边缘开始。在圆形和正方形中，细胞运动遵循荷包缝合机制/混合模式。因此，本研究表明，为了更快地愈合伤口，可能发现沿着伤口边缘的细胞迁移是有益的，并且以工程蛋白水凝胶形式的外部愈合剂可以相应地加速愈合。
