Marashi Sayed Mahdi, Mostafa Aliehossadat, Shoja Zabihollah, Nejati Ahmad, Shahmahmoodi Shohreh, Mollaei-Kandelous Yaghoub, Sahraian Mohammad Ali, Jalilvand Somayeh
1Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, 14155 Iran.
2Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
Virusdisease. 2018 Dec;29(4):540-543. doi: 10.1007/s13337-018-0481-1. Epub 2018 Aug 10.
Several studies reported a complex interplay between viral infections and neural cells leading to multiple sclerosis. A role for some viral infections has been proposed in MS. In this study, DNA sequences of human herpesvirus 8 (HHV-8) were searched in the peripheral blood of 54 patients with multiple sclerosis and 130 healthy subjects using nested-PCR assay to amplify ORF26 locus. Furthermore, HHV-8 positive samples were subjected to a nested-PCR to amplify K1 gene of HHV-8 followed by direct nucleotide sequencing. HHV-8 genome was detected in 18.5% (10/54) and 3% (4/130) of MS patients and controls, respectively, and the difference reached statistically significant level ( = 0.0017). Genotyping analysis revealed that genotype C was common (88.9%) in all study subjects, followed by genotype A. Our results showed higher detection of HHV-8 DNA in MS patients than control group.
多项研究报告了病毒感染与神经细胞之间复杂的相互作用，这种相互作用会导致多发性硬化症。已有人提出某些病毒感染在多发性硬化症中起作用。在本研究中，使用巢式聚合酶链反应（nested-PCR）分析法扩增ORF26基因座，在54例多发性硬化症患者和130名健康受试者的外周血中搜索人类疱疹病毒8（HHV-8）的DNA序列。此外，对HHV-8阳性样本进行巢式PCR以扩增HHV-8的K1基因，随后进行直接核苷酸测序。在多发性硬化症患者和对照组中，HHV-8基因组的检测率分别为18.5%（10/54）和3%（4/130），差异达到统计学显著水平（P = 0.0017）。基因分型分析显示，在所有研究对象中，基因型C最为常见（88.9%），其次是基因型A。我们的结果表明，多发性硬化症患者中HHV-8 DNA的检测率高于对照组。
