Laboratorio de Microbiología Sanitaria, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Marcelino García Barragán No. 1421, 44430, Guadalajara, Jalisco, Mexico.
Departamento de Ciencias Biomédicas, Centro Universitario de Tonalá, Universidad de Guadalajara, Av. Periférico Norte No. 555, 48525, Tonalá, Jalisco, Mexico.
BMC Complement Altern Med. 2018 Apr 10;18(1):127. doi: 10.1186/s12906-018-2180-2.
Therapeutic use of leaves of M. oleifera has been evaluated in diabetes because of its possible capacity to decrease blood glucose and lipids concentration after ingestion, as result of the polyphenols content and others compounds. Nevertheless most results have been obtain from leaf extract, therefore this study would use leaf powder as the regular way of consumption of population to know effects over toxicity glucose, triglycerides, cholesterol, corporal weight, and predominant groups of microbiota.
Powdered leaf was administrated in different doses to know toxicity and genotoxicity using LD50 and micronuclei assay. Hyperglycemia was induced by alloxan on Sprague Dawley rats. Glucose and body weight were measured once a week meanwhile cholesterol and triglycerides were analyzed at the end of the study by commercial kits. Different organs were examined by hematoxylin-eosin technique. Lactic acid bacteria and Enterobacteriaceae were enumerated from stool samples.
The tested doses revealed no lethal dose and no significant differences in genotoxicity parameter. The consumption of the leaves showed a hypoglycemic effect (< 250 mg/dL in diabetic M. oleifera treated group), however in corporal weight showed an increased (> 30 g over no M. oleifera treated groups). There was no change in enumeration of lactic acid bacteria (8.4 CFU/g) but there were differences in the predominance of type of lactobacillus and enterobacteria enumeration.
These results help to increase information over the most popular use of M. oleifera and its safety. However there are needed more studies over the hypoglycemic mechanisms and effects over intestinal microbiota.
由于多榔菊叶中富含多酚类化合物和其他化合物，因此其在摄入后可能具有降低血糖和血脂浓度的能力，故其在糖尿病治疗中的应用价值已得到评估。然而，大多数研究结果都来自于叶提取物，因此本研究将使用叶粉作为人群的常规食用方式，以了解其对血糖、甘油三酯、胆固醇、体重和主要菌群的影响。
通过测定半数致死量（LD50）和微核试验，用不同剂量的叶粉来评估其毒性和遗传毒性。用四氧嘧啶诱导 Sprague Dawley 大鼠产生高血糖。每周测量一次血糖和体重，同时在研究结束时使用商业试剂盒分析胆固醇和甘油三酯。用苏木精-伊红技术检查不同的器官。从粪便样本中计数乳酸菌和肠杆菌科。
测试剂量未显示致死剂量，也未显示遗传毒性参数有显著差异。叶粉的食用显示出降血糖作用（糖尿病多榔菊治疗组<250mg/dL），但体重增加（多榔菊治疗组比未治疗组增加>30g）。乳酸菌计数（8.4 CFU/g）没有变化，但乳酸菌和肠杆菌科的优势类型的计数存在差异。
这些结果有助于增加关于多榔菊最常见用途及其安全性的信息。然而，还需要更多关于其降血糖机制和对肠道菌群影响的研究。
