利用重复DNA探针鉴别恶性疟原虫克隆

Differentiation of Plasmodium falciparum clones by means of a repetitive DNA probe.

作者信息

Fucharoen S, Tirawanchai N, Wilairat P, Panyim S, Thaithong S

机构信息

Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.

出版信息

Trans R Soc Trop Med Hyg. 1988;82(2):209-11. doi: 10.1016/0035-9203(88)90413-0.

DOI:10.1016/0035-9203(88)90413-0

PMID:3055455

Abstract

A recombinant DNA probe, pBRK1-14, was constructed by inserting a repetitive DNA fragment of 753 base pairs obtained from Plasmodium falciparum, isolate K1, into the EcoRI/PstI cloning sites of pBR322. This probe could discriminate between different parasite clones derived from a single isolate by hybridization with Southern genomic blots.

摘要

通过将从恶性疟原虫K1分离株获得的753个碱基对的重复DNA片段插入pBR322的EcoRI/PstI克隆位点，构建了重组DNA探针pBRK1-14。该探针可通过与Southern基因组印迹杂交来区分源自单个分离株的不同寄生虫克隆。