Ge Sheng, Ye Pei, Li Guo-Yang, Fu Yi-Fu, Zhou Qian, Huang Fan, Wang Xiang, Wang Wen-Mei
1 Department of Oral Medicine, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, China.
2 Department of Endodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, China.
Toxicol Ind Health. 2019 Feb;35(2):109-118. doi: 10.1177/0748233718817192. Epub 2018 Dec 17.
Cigarette smoking is an established risk factor for some oral diseases. As an essential fluid in the oral cavity, saliva is crucial to maintain oral health. Relative to active smoking, there are very few studies assessing the effect of passive smoking on salivary cytokines levels. In the present study, we established the rat models by the means of the intraoral cigarette smoking or whole body cigarette smoke exposure to simulate human active or passive smoking, respectively. The effects of active or passive smoking on salivary cytokines levels were assessed by using ProcartaPlex multiplex immunoassays. The results of the current study indicated that both active and passive smoking diminished the body weights of rats and increased the levels of some blood counts. Intriguingly, active smoking enhanced the salivary levels of IL-6 and IL-12 p70 and passive smoking elevated the salivary IL-6 level. Moreover, active smoking appeared to have a more prominent activation effect on the salivary IL-6 level. It was noted that active or passive smoking had no significant effect on the salivary IFN-γ level. Active or passive smoking could have potential effects on the salivary levels of some pro-inflammatory cytokines.
吸烟是某些口腔疾病公认的风险因素。唾液作为口腔中的一种重要液体，对维持口腔健康至关重要。相对于主动吸烟，评估被动吸烟对唾液细胞因子水平影响的研究非常少。在本研究中，我们分别通过口腔内吸烟或全身暴露于香烟烟雾的方式建立大鼠模型，以模拟人类主动吸烟或被动吸烟。使用ProcartaPlex多重免疫测定法评估主动或被动吸烟对唾液细胞因子水平的影响。当前研究结果表明，主动吸烟和被动吸烟均会降低大鼠体重并提高一些血细胞计数水平。有趣的是，主动吸烟会提高唾液中IL-6和IL-12 p70的水平，而被动吸烟会提高唾液中IL-6的水平。此外，主动吸烟似乎对唾液IL-6水平具有更显著的激活作用。值得注意的是，主动吸烟或被动吸烟对唾液IFN-γ水平均无显著影响。主动或被动吸烟可能会对某些促炎细胞因子的唾液水平产生潜在影响。
