Amity Institute of Biotechnology, Amity University Uttar Pradesh Lucknow Campus, Lucknow, Uttar Pradesh, 226028, India.
Department of Oral Pathology, King George Medical University, Lucknow, Uttar Pradesh, India.
Eur Arch Otorhinolaryngol. 2021 Oct;278(10):4011-4019. doi: 10.1007/s00405-021-06620-z. Epub 2021 Jan 27.
Our study aims to determine the influence of smoking or tobacco chewing and the association of Interleukin 6 (IL-6) polymorphism, where G is substituted by A at the position - 596 (IL-6 - 596 G/A) and substitution of G by cytosine (C) at position - 572 (IL-6 - 572 G/C) on the susceptibility of precancerous oral lesions and oral cancer.
The participants consisted of 250 subjects among which 75 were suffering from oral cancer, 75 subjects with precancerous oral lesions and 100 were healthy controls. Single-nucleotide polymorphism study (SNP) was done by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).
IL-6 - 596 G/A SNP revealed genotypes, GG, and GA in subjects with precancerous oral lesions and oral cancer, and AA genotype was not found in any subject. IL-6 - 596 G/A was strongly associated with oral precancerous lesions but not with oral cancer. The present study reports that smokers carrying GA for IL-6 - 596 G/A were at several folds higher risk of developing oral precancerous lesions. Smokers with GC and CC for IL-6 - 572 G/C were at higher risk of developing oral precancerous lesions. No significant interaction was observed between these habits and IL-6 - 596 G/A and IL-6 - 572 G/C SNP with oral cancer.
The interaction of variant A allele of IL-6 - 596 G/A and C allele of IL-6 - 572 G/C polymorphism with smoking and increases the risk of oral precancerous lesions. Tobacco chewing was not related with IL-6 - 596 G/A or IL-6 - 572 G/C in oral precancerous lesions or oral cancer.
The study will help to determine the susceptibility of individuals with smoking or chewing habits to the development of oral precancerous lesion and oral cancer by monitoring the IL-6 SNPs which can be used as a biomarker for risk determination.
本研究旨在探讨吸烟或咀嚼烟草以及白细胞介素 6（IL-6）基因多态性（其中 G 被位置 -596 的 A 取代[IL-6-596 G/A]，以及 G 被位置 -572 的胞嘧啶（C）取代[IL-6-572 G/C]）对癌前口腔病变和口腔癌易感性的影响。
参与者包括 250 名受试者，其中 75 名患有口腔癌，75 名患有癌前口腔病变，100 名是健康对照。单核苷酸多态性研究（SNP）通过聚合酶链反应和限制性片段长度多态性（PCR-RFLP）进行。
IL-6-596 G/A SNP 显示基因型 GG 和 GA 存在于癌前口腔病变和口腔癌患者中，而 AA 基因型在任何受试者中均未发现。IL-6-596 G/A 与口腔癌前病变密切相关，但与口腔癌无关。本研究报告称，携带 IL-6-596 G/A 的 GA 基因型的吸烟者患口腔癌前病变的风险增加数倍。携带 IL-6-572 G/C 的 GC 和 CC 基因型的吸烟者患口腔癌前病变的风险更高。未观察到这些习惯与 IL-6-596 G/A 和 IL-6-572 G/C SNP 与口腔癌之间存在显著的相互作用。
IL-6-596 G/A 变体 A 等位基因与 IL-6-572 G/C 多态性与吸烟的相互作用增加了口腔癌前病变的风险。咀嚼烟草与 IL-6-596 G/A 或 IL-6-572 G/C 多态性在口腔癌前病变或口腔癌中无关。
该研究将有助于通过监测 IL-6 SNP 来确定具有吸烟或咀嚼习惯的个体发生口腔癌前病变和口腔癌的易感性，这些 SNP 可以作为风险确定的生物标志物。
