基于硅酸钙的根管封闭剂的抗炎和成骨作用。

Anti-inflammatory and Osteogenic Effects of Calcium Silicate-based Root Canal Sealers.

机构信息

Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea.

Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul, Korea.

出版信息

J Endod. 2019 Jan;45(1):73-78. doi: 10.1016/j.joen.2018.09.006.

DOI:10.1016/j.joen.2018.09.006

PMID:30558800

Abstract

INTRODUCTION

An ideal root canal sealer creates a bacteria-resistant seal and exhibits antimicrobial activity, biocompatibility, and osteoconductivity. The aim of this study was to assess the effects of 3 root canal sealers on cell viability, inflammatory response, and osteogenic potential in MC3T3-E1 cells.

METHODS

AH Plus (Dentsply Caulk, Milford, DE), MTA Fillapex (Angelus Solucxoes Odontologicas, Londrina, Brazil), and EndoSequence BC (Brasseler, Savannah, GA) were mixed according to the manufacturer's instructions, and samples were prepared as extraction media (final dilution: 1/10). Lipopolysaccharide (LPS) (100 ng/mL) treatment was used to induce an inflammatory response in this study. Cell viability was evaluated using the Water soluble tetrazolium-1 (WST-1) assay. The levels of inflammatory mediators (interleukin 6 and tumor necrosis factor alpha) and osteogenic marker genes (ALP and OCN) were measured by reverse-transcription polymerase chain reaction and real-time polymerase chain reaction. Osteogenic potential was evaluated by alkaline phosphatase staining and alizarin red staining.

RESULTS

Calcium silicate-based sealers such as MTA Fillapex and EndoSequence BC showed strong cell viability compared with AH Plus. AH Plus, MTA Fillapex, and EndoSequence BC decreased the levels of LPS-induced inflammatory mediators (P < .05). The expression of osteogenic marker genes, alkaline phosphatase activity, and mineralized nodule formation decreased with LPS treatment. However, AH Plus and calcium silicate-based sealers increased the osteogenic potential reduced by LPS treatment (P < .05).

CONCLUSIONS

Calcium silicate-based sealers exhibit anti-inflammatory effects and induce osteogenic differentiation in MC3T3-E1 cells.

摘要

简介

理想的根管封闭剂应具备抗细菌密封性能，并具有抗菌活性、生物相容性和骨诱导性。本研究旨在评估 3 种根管封闭剂对 MC3T3-E1 细胞活力、炎症反应和成骨潜能的影响。

方法

根据制造商的说明混合 AH Plus（登士柏，米尔福德，DE）、MTA Fillapex（安格卢斯 Solucxoes Odontologicas，朗德里纳，巴西）和 EndoSequence BC（布莱斯勒，萨凡纳，GA），并将样本制备为提取介质（最终稀释度：1/10）。本研究中使用脂多糖（LPS）（100ng/mL）处理诱导炎症反应。通过水溶性四唑盐-1（WST-1）测定法评估细胞活力。通过逆转录聚合酶链反应和实时聚合酶链反应测量炎症介质（白细胞介素 6 和肿瘤坏死因子-α）和成骨标志物基因（碱性磷酸酶和骨钙素）的水平。通过碱性磷酸酶染色和茜素红染色评估成骨潜能。

结果

与 AH Plus 相比，基于硅酸钙的封闭剂如 MTA Fillapex 和 EndoSequence BC 具有更强的细胞活力。AH Plus、MTA Fillapex 和 EndoSequence BC 降低了 LPS 诱导的炎症介质水平（P<0.05）。LPS 处理降低了成骨标志物基因的表达、碱性磷酸酶活性和矿化结节形成。然而，AH Plus 和基于硅酸钙的封闭剂增加了 LPS 处理降低的成骨潜能（P<0.05）。