He Hongjian, Xu Bing
Department of Chemistry, Brandeis University, 415 South Street, Waltham, MA 02454, USA.
Bull Chem Soc Jpn. 2018;91(6):900-906. doi: 10.1246/bcsj.20180038. Epub 2018 Mar 10.
Instructed-assembly (iAssembly or iA) refers to the formation of ordered superstructures of molecules as the consequence of at least one trigger event (e.g., a reaction or a ligand-receptor interaction). As a biomimetic process that transforms from an equilibrium to another equilibrium, iA has emerging as a powerful approach to provide spatiotemporal control for a range of potential biomedical applications, including molecular imaging, cancer therapy, and tissue engineering. This account introduces the general concept of iA in the context of cells and illustrates how to achieve iA for applications. By mainly describing the representative examples of iA and its applications in complex environment, such as cells or animals, and providing the perspectives of the future development of iA, we intend to show that, as a process that bridges self-assembly and self-organization, iA offers chemists a facile mean to explore the emergent properties of molecular assemblies and the dynamics of molecular processes to control cell fate. Particularly, iA promises many wonderful surprises and useful applications in physical and/or life sciences when multiple processes (e.g., self-assembly, instructed-assembly, and self-organization) are taking place simultaneously.
指令组装（iAssembly或iA）是指由于至少一个触发事件（例如反应或配体-受体相互作用）而形成分子的有序超结构。作为一种从一种平衡转变为另一种平衡的仿生过程，iA已成为一种强大的方法，可为一系列潜在的生物医学应用提供时空控制，包括分子成像、癌症治疗和组织工程。本综述在细胞背景下介绍了iA的一般概念，并说明了如何实现用于应用的iA。通过主要描述iA在复杂环境（如细胞或动物）中的代表性实例及其应用，并提供iA未来发展的展望，我们旨在表明，作为一种连接自组装和自组织的过程，iA为化学家提供了一种简便的手段，以探索分子组装的涌现特性和控制细胞命运的分子过程动力学。特别是，当多个过程（例如自组装、指令组装和自组织）同时发生时，iA在物理和/或生命科学中有望带来许多奇妙的惊喜和有用的应用。
