Yuksel Nilay, Tanriverdi Burak, Ipteç Betul, Erel Ozcan
Ophthalmology Department, Ankara Ataturk Training and Research Hospital , Ankara , Turkey.
Medical Faculty, Ophthalmology Department, Yıldırım Beyazıt University , Ankara , Turkey.
Orbit. 2019 Oct;38(5):370-375. doi: 10.1080/01676830.2018.1553191. Epub 2018 Dec 18.
: To evaluate thiol-disulfide homeostasis (TDH) and its relationship with clinical findings in patients with Graves' ophthalmopathy (GO). : This study included 52 patients with GO and 34 healthy controls. Tests of TDH were conducted using the novel automated spectrophotometric method. Clinical activity score (CAS) and ophthalmopathy index were evaluated and relationships with native thiol, disulfide levels and disulfide/native thiol % ratios were analyzed. : The mean plasma native thiol levels in GO patients were significantly lower than that of normal controls ( = 0.013) . The mean plasma disulfide levels and disulfide/native thiol % ratio were found to be significantly higher in GO patients than in controls ( = 0.041, = 0.022; respectively). The mean native thiol levels of active GO patients ( = 24) were significantly lower than that of patients with inactive GO ( = 28) ( = 0.044). The mean disulfide levels and disulfide/native thiol % ratios of active GO patients were significantly higher than that of patients with inactive GO ( = 0.034, = 0.001; respectively). There was a negative correlation between native thiol and CAS ( = -0.532, = 0.001) and a positive correlation between disulfide/native thiol % ratio and CAS ( = 0.601, < 0.001). : The impairment of TDH indicates the presence of oxidative stress in moderate-to-severe GO, particularly in active GO and smokers.
评估格雷夫斯眼病（GO）患者的硫醇 - 二硫键稳态（TDH）及其与临床 findings 的关系。：本研究纳入了52例GO患者和34名健康对照者。使用新型自动分光光度法进行TDH检测。评估临床活动评分（CAS）和眼病指数，并分析其与天然硫醇、二硫键水平及二硫键/天然硫醇百分比比值的关系。：GO患者的平均血浆天然硫醇水平显著低于正常对照者（P = 0.013）。发现GO患者的平均血浆二硫键水平和二硫键/天然硫醇百分比比值显著高于对照者（P = 0.041，P = 0.022；分别）。活动期GO患者（n = 24）的平均天然硫醇水平显著低于非活动期GO患者（n = 28）（P = 0.044）。活动期GO患者的平均二硫键水平和二硫键/天然硫醇百分比比值显著高于非活动期GO患者（P = 0.034，P = 0.001；分别）。天然硫醇与CAS之间存在负相关（r = -0.532，P = 0.001），二硫键/天然硫醇百分比比值与CAS之间存在正相关（r = 0.60l，P < 0.001）。：TDH的损害表明中重度GO，特别是活动期GO和吸烟者存在氧化应激。
