Lin Chunhui, Wan Jiajia, Su Yong, Zhu Weiyun
Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing 210095, China.
Metabolites. 2018 Dec 6;8(4):89. doi: 10.3390/metabo8040089.
We investigated the effects of early intervention with maternal fecal microbiota and antibiotics on gut microbiota and the metabolites. Five litters of healthy neonatal piglets (Duroc × Landrace × Yorkshire, nine piglets in each litter) were used. Piglets in each litter were orally treated with saline (CO), amoxicillin treatment (AM), or maternal fecal microbiota transplantation (MFMT) on days 1⁻6, with three piglets in each treatment. Results were compared to the CO group. MFMT decreased the relative abundances of and in the colon on day 7, whereas the abundance of increased, and the abundance of in the stomach reduced on day 21. AM reduced the abundance of in the stomach on day 7 and reduced the abundances of and in the ileum and colon on day 21, respectively. The metabolite profile indicated that MFMT markedly influenced carbohydrate metabolism and amino acid (AA) metabolism on day 7. On day 21, carbohydrate metabolism and AA metabolism were affected by AM. The results suggest that MFMT and AM discriminatively modulate gastrointestinal microflora and alter the colonic metabolic profiles of piglets and show different effects in the long-term. MFMT showed a location-specific influence on the gastrointestinal microbiota.
我们研究了母体粪便微生物群和抗生素早期干预对肠道微生物群及代谢产物的影响。使用了5窝健康新生仔猪（杜洛克×长白×约克夏，每窝9头仔猪）。在第1至6天，每窝仔猪分别口服生理盐水（CO组）、阿莫西林治疗（AM组）或母体粪便微生物群移植（MFMT组），每组3头仔猪。将结果与CO组进行比较。MFMT在第7天降低了结肠中 和 的相对丰度，而 在第21天丰度增加，胃中 的丰度降低。AM在第7天降低了胃中 的丰度，在第21天分别降低了回肠和结肠中 和 的丰度。代谢物谱表明，MFMT在第7天显著影响碳水化合物代谢和氨基酸（AA）代谢。在第21天，碳水化合物代谢和AA代谢受AM影响。结果表明，MFMT和AM对仔猪胃肠道微生物群有差异调节作用，并改变其结肠代谢谱，且长期效果不同。MFMT对胃肠道微生物群有位置特异性影响。
