Xu Rongying, Wan Jiajia, Lin Chunhui, Su Yong
Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing 210095, China.
Antibiotics (Basel). 2020 Jan 18;9(1):35. doi: 10.3390/antibiotics9010035.
This study aimed to investigate the effects of early intervention with antibiotics and maternal fecal microbiota on ileal morphology and barrier function, and transcriptomic profiling in neonatal piglets. Piglets in the amoxicillin (AM), fecal microbiota transplantation (FMT), and control (CO) groups were orally administrated with amoxicillin solution (6.94 mg/mL), maternal fecal microbiota suspension [>10 colony forming unit (CFU)/mL], and physiological saline, respectively. Compared with the CO group, early intervention with AM or FMT significantly decreased ileal crypt depth on day 7 and altered gene expression profiles in ileum on days 7 and 21, and especially promoted the expression of chemokines (, and ) involved in the toll-like receptor signaling pathway on day 21. FMT changed major immune activities from B cell immunity on day 7 to T cell immunity on day 21 in the ileum. On the other hand, both AM and FMT predominantly downregulated the gene expression of toll-like receptor 4 (). In summary, both early interventions modulated intestinal barrier function and immune system in the ileum with a low impact on ileal morphology and development.
本研究旨在探讨抗生素早期干预和母体粪便微生物群对新生仔猪回肠形态、屏障功能及转录组图谱的影响。阿莫西林（AM）组、粪便微生物群移植（FMT）组和对照组（CO）的仔猪分别口服阿莫西林溶液（6.94 mg/mL）、母体粪便微生物群悬液[>10菌落形成单位（CFU）/mL]和生理盐水。与CO组相比，AM或FMT早期干预在第7天显著降低了回肠隐窝深度，并在第7天和第21天改变了回肠的基因表达谱，尤其是在第21天促进了参与Toll样受体信号通路的趋化因子（、和）的表达。FMT使回肠的主要免疫活动从第7天的B细胞免疫转变为第21天的T细胞免疫。另一方面，AM和FMT均主要下调Toll样受体4（）的基因表达。总之，两种早期干预均调节了回肠的肠道屏障功能和免疫系统，对回肠形态和发育的影响较小。
