Wan Jia-Jia, Lin Chun-Hui, Ren Er-Du, Su Yong, Zhu Wei-Yun
Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, China.
Front Physiol. 2019 Feb 27;10:171. doi: 10.3389/fphys.2019.00171. eCollection 2019.
The establishment of a stable bacterial flora in early life is associated with host metabolism. Studies of fecal microbiota transplantation (FMT) and antibiotics on neonatal pig mainly focused on intestinal development and mucosal immunity, but the information on metabolism is lacking. The objective of this study was to investigate the responses of metabolome and transcriptome in the livers of neonatal piglets that were orally inoculated with maternal fecal bacteria suspension and amoxicillin (AM) solution. Five litters of Duroc × Landrace × Yorkshire neonatal piglets were used as five replicates and nine piglets in each litter were randomly assigned to the control (CO), AM or FMT groups. Neonatal piglets in three groups were fed with 3 mL saline (0.9%), AM solution (6.94 mg/mL) or fecal bacteria suspension (>10/mL), respectively, on days 1-6. At the age of 7 and 21 days, one piglet from each group in each litter was sacrificed, and the serum and liver were collected for analysis. The RNA sequencing analysis showed that the mRNA expressions of () () () () and () were downregulated ( < ) by AM on day 7, and that the mRNA expressions of () and were downregulated () by FMT on day 7. GC-MS analysis showed that AM and FMT treatments mainly affected fatty acid metabolism and amino acid metabolism on days 7 and 21. AM and FMT both reduced () the blood levels of triglycerides and low density lipoprotein cholesterol (LDL-C) on day 7. AM reduced ) the blood level of cholesterol on day 21, and FMT reduced the blood levels of cholesterol, triglycerides and LDL-C on day 21. These results indicate that early intervention with FMT or AM can reduce fatty acid oxidative catabolism and amino acid biosynthesis of neonatal piglets, which provides a reference for regulation host metabolism through early intervention in animal production and even human health.
生命早期稳定的细菌菌群的建立与宿主代谢相关。关于新生仔猪粪便微生物群移植（FMT）和抗生素的研究主要集中在肠道发育和黏膜免疫方面，但缺乏关于代谢的信息。本研究的目的是调查口服接种母体粪便细菌悬液和阿莫西林（AM）溶液的新生仔猪肝脏中代谢组和转录组的反应。选取5窝杜洛克×长白×约克夏新生仔猪作为5个重复，每窝9头仔猪随机分为对照组（CO）、AM组或FMT组。在第1至6天，三组新生仔猪分别喂食3 mL生理盐水（0.9%）、AM溶液（6.94 mg/mL）或粪便细菌悬液（>10/mL）。在7日龄和21日龄时，每窝每组处死1头仔猪，收集血清和肝脏进行分析。RNA测序分析表明，AM在第7天使（）（）（）（）和（）的mRNA表达下调（<），FMT在第7天使（）和的mRNA表达下调（）。气相色谱-质谱联用（GC-MS）分析表明，AM和FMT处理在第7天和第21天主要影响脂肪酸代谢和氨基酸代谢。AM和FMT在第7天都降低了（）甘油三酯和低密度脂蛋白胆固醇（LDL-C）的血液水平。AM在第21天降低了（）胆固醇的血液水平，FMT在第21天降低了胆固醇、甘油三酯和LDL-C的血液水平。这些结果表明，FMT或AM的早期干预可降低新生仔猪的脂肪酸氧化分解代谢和氨基酸生物合成，这为通过早期干预动物生产甚至人类健康来调节宿主代谢提供了参考。
