Courtois J, Courtois B, Guillaume J
Laboratoire de Microbiologie, U.F.R. de Biologie, Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France.
J Bacteriol. 1988 Dec;170(12):5925-7. doi: 10.1128/jb.170.12.5925-5927.1988.
Transformation of R factor RP4 and its derivative pRK290 from Escherichia coli to Rhizobium meliloti is reported. The efficiency of transformation was in the range of 10(-5) per viable cell. In addition, chromosomal DNA prepared from one R. meliloti strain resistant to streptomycin was transferred to the isoleucine-valine-requiring mutant susceptible to streptomycin.
据报道,R因子RP4及其衍生物pRK290可从大肠杆菌转移至苜蓿根瘤菌。转化效率为每存活细胞10(-5) 。此外,从一株对链霉素有抗性的苜蓿根瘤菌菌株制备的染色体DNA被转移至对链霉素敏感的异亮氨酸-缬氨酸需求型突变体。