Julliot J S, Boistard P
Mol Gen Genet. 1979 Jun 20;173(3):289-98. doi: 10.1007/BF00268639.
RP4-prime plasmids containing chromosomal fragments of either Escherichia coli or Rhizobium meliloti were constructed in vitro. When introduced into E. coli or R. meliloti respectively, they promoted a polarized transfer of the chromosome as demonstrated either by the gradient of transfer of various markers or by the study of the genetic constitution of recombinants. In E. coli, mobilization was shown to be dependent upon the presence of a functional rec A system. Inheritance of markers was due to their integration into the chromosome of the recipient as shown by the need for a functional rec A system in the recipient E. coli or by mobilization of recessive markers in R. meliloti. The system described could be applied to genetic mapping in any Gram negative bacteria.
含有大肠杆菌或苜蓿根瘤菌染色体片段的RP4-prime质粒在体外构建。当分别导入大肠杆菌或苜蓿根瘤菌时，它们促进了染色体的极性转移，这通过各种标记的转移梯度或重组体遗传组成的研究得以证明。在大肠杆菌中，转移显示依赖于功能性rec A系统的存在。标记的遗传是由于它们整合到受体的染色体中，这在受体大肠杆菌中对功能性rec A系统的需求或苜蓿根瘤菌中隐性标记的转移中得到体现。所描述的系统可应用于任何革兰氏阴性细菌的基因定位。
