Querzoli P, Marchetti E, Bagni A, Marzola A, Fabris G, Nenci I
Istituto di Anatomia e Istologia Patologica, Università di Ferrara, Italy.
Breast Cancer Res Treat. 1988 Sep;12(1):23-30. doi: 10.1007/BF01805736.
Expression of the p21 product of the ras gene family was investigated in a series of 142 infiltrating primary breast tumors by two specific ras p21 monoclonal antibodies with an immunocytochemical technique. The majority of tumors demonstrated a varying number of positive cells. A significant association between p21 expression and tumor histotype was found: among ductal carcinomas the comedo variety was always positive; conversely, lobular tumors were more frequently negative. Nodal status was recorded for all patients. A significant difference was found in nodal status with respect to p21 expression: tumors with more than 50% positive cells were more often N+. Estrogen receptor (ER) status was determined in 77 tumors. Tumors with higher levels of p21 contained a high percentage of estrogen receptor positive cells. The present results show that p21 expression in human breast cancer could be a marker of tumor aggressiveness and might thus improve the predictive power of known prognostic factors such as estrogen receptor and nodal status.
采用免疫细胞化学技术，运用两种特异性的ras p21单克隆抗体，对142例原发性浸润性乳腺癌进行研究，以检测ras基因家族p21产物的表达情况。大多数肿瘤显示出数量不等的阳性细胞。研究发现p21表达与肿瘤组织学类型之间存在显著关联：在导管癌中，粉刺样癌总是呈阳性；相反，小叶肿瘤更常呈阴性。记录了所有患者的淋巴结状态。在淋巴结状态方面，发现p21表达存在显著差异：阳性细胞超过50%的肿瘤更常出现N+。在77例肿瘤中测定了雌激素受体（ER）状态。p21水平较高的肿瘤含有高比例的雌激素受体阳性细胞。目前的结果表明，人乳腺癌中的p21表达可能是肿瘤侵袭性的一个标志物，因此可能提高已知预后因素如雌激素受体和淋巴结状态的预测能力。
