Bai Ge, Li Hui, Ge Yansong, Zhang Qianzhen, Zhang Jiantao, Chen Mingzi, Liu Tao, Wang Hongbin
Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
J Vet Res. 2018 Dec 10;62(3):395-403. doi: 10.2478/jvetres-2018-0056. eCollection 2018 Sep.
The purpose of this study was to investigate the protective effect of hydrogen-rich saline (HRS) against liver ischaemia-reperfusion combined resection injury.
Eighteen miniature pigs were randomly divided into three groups: a sham operated group (sham group, laparoscopic liver ischaemia-reperfusion combined resection injury group (IRI group), and a hydrogen-rich saline intervention group (IRI + HRS group). Samples of hepatic tissue and serum were collected at the time of reperfusion and then 3 h, 1 d, and 3 d post reperfusion. Liver function, oxidative stress, autophagy-related mRNA genes, and protein expression were evaluated. Changes in cell and tissue ultrastructure were examined by transmission electron microscopy.
Compared with the sham group, the level of autophagy of hepatocytes increased in the IRI and IRI + HRS groups, corresponding to high oxidative stress and severe liver function injury. Liver function, antioxidant content, autophagy levels, and liver injury were improved after intervention with HRS in the IRI + HRS group compared with the IRI group.
Intervention with hydrogen-rich saline could exert a protective effect against liver ischaemia-reperfusion combined resection injury through the reduction of oxidative stress and hepatocyte autophagy.
本研究旨在探讨富氢盐水（HRS）对肝脏缺血再灌注联合切除损伤的保护作用。
18只小型猪随机分为三组：假手术组（假手术组）、腹腔镜肝脏缺血再灌注联合切除损伤组（IRI组）和富氢盐水干预组（IRI + HRS组）。在再灌注时以及再灌注后3小时、1天和3天采集肝组织和血清样本。评估肝功能、氧化应激、自噬相关mRNA基因和蛋白质表达。通过透射电子显微镜检查细胞和组织超微结构的变化。
与假手术组相比，IRI组和IRI + HRS组肝细胞自噬水平升高，同时伴有高氧化应激和严重肝功能损伤。与IRI组相比，IRI + HRS组在接受HRS干预后，肝功能、抗氧化剂含量、自噬水平和肝损伤均得到改善。
富氢盐水干预可通过降低氧化应激和肝细胞自噬对肝脏缺血再灌注联合切除损伤发挥保护作用。
