采用气相色谱-火焰光度检测法测定草甘膦及其代谢物氨甲基膦酸。

A method for determining glyphosate and its metabolite aminomethyl phosphonic acid by gas chromatography-flame photometric detection.

机构信息

Department of Sanitary Inspection, School of Public Health, Hebei Medical University, Shijiazhuang, 050017, China.

出版信息

J Chromatogr A. 2019 Mar 29;1589:116-121. doi: 10.1016/j.chroma.2018.12.039. Epub 2018 Dec 20.

DOI:10.1016/j.chroma.2018.12.039

Abstract

As a globally popular herbicide, glyphosate (GLY) and its metabolite aminomethylphosphonic acid (AMPA) pose potential hazards to the ecological environment. In this study, a sensitive and reliable method for detecting GLY and AMPA was utilized to facilitate exposure risk assessment of the analytes in environmental systems such as water and soil. GLY and AMPA were extracted from the sample using a solid-phase extraction (SPE) procedure, derivatized by heptafluorobutyric anhydride and heptafluorobutanol, and detected by gas chromatography-flame photometric detection (GC-FPD). The linearities of GLY and AMPA in the range of 10-1000 ng/mL were good (r=0.9998, r=0.9991), and the limits of quantitation (LOQ) for GLY and AMPA were 0.37 and 0.81 ng/mL, respectively. The method has been successfully applied for detecting GLY and AMPA in water, soil and monitoring the degradation of GLY under different environmental conditions. Simulated migration characteristics of GLY and AMPA in soil were investigated for evaluating the potential hazards of GLY and AMPA to the ecological environment.

摘要

作为一种全球流行的除草剂，草甘膦（GLY）及其代谢物氨甲基膦酸（AMPA）对生态环境构成潜在危害。在本研究中，采用了一种灵敏可靠的方法来检测 GLY 和 AMPA，以促进对环境系统（如水和土壤）中这些分析物的暴露风险评估。通过固相萃取（SPE）程序从样品中提取 GLY 和 AMPA，用七氟丁酰酐和七氟丁醇衍生化，并用气相色谱-火焰光度检测（GC-FPD）进行检测。GLY 和 AMPA 的线性范围为 10-1000ng/mL（r=0.9998，r=0.9991），GLY 和 AMPA 的定量限（LOQ）分别为 0.37 和 0.81ng/mL。该方法已成功应用于检测水、土壤中的 GLY 和 AMPA，并监测不同环境条件下 GLY 的降解情况。研究了 GLY 和 AMPA 在土壤中的模拟迁移特征，以评估 GLY 和 AMPA 对生态环境的潜在危害。