Lee Ok Joo, Kim Jung-Ho, Moon Bo Mi, Chao Janet Ren, Yoon Jaeho, Ju Hyung Woo, Lee Jung Min, Park Hyun Jung, Kim Dong Wook, Kim Seung Ju, Park Hae Sang, Park Chan Hum
1Nano-Bio Regenerative Medical Institute, Hallym University, 1 Hallymdaehak-gil, Chuncheon, 24252 Korea.
2School of Medicine, George Washington University, Washington, D.C., USA.
Tissue Eng Regen Med. 2016 Jun 9;13(3):218-226. doi: 10.1007/s13770-016-9058-5. eCollection 2016 Jun.
Hydrocolloid dressings have been developed for many types of wound healing. In particular, dressing is a critical component in the successful recover of burn injuries, which causes a great number of people to not only suffer from physical but also psychological and economic anguish each year. Additionally, silk fibroin is the safest material for tissue engineering due to biocompatibility. In this study, we fabricated hydrocolloid dressings incorporating silk fibroin nanoparticles to enhance the efficacy of hydrocolloid dressing and then use this silk fibroin nanoparticle hydrocolloid dressing (SFNHD) in animal models to treat burn wounds. The structures and properties of SFNHD were characterized using tensile strength and Cell Counting Kit-8 assay. The results indicated the structural stability and the cellular biocompatibility of the hydrocolloid dressing suggesting that SFNHD can be applied to the treatment of wounds. To demonstrate the capacity of a silk fibroin hydrocolloid dressing to treat burn wounds, we compared SFNHD to gauze and Neoderm, a commercially available dressing. This study clearly demonstrated accelerated wound healing with greater wound structural integrity and minimal wound size after treatment with SFNHD. These observations indicate that SFNHD may be an improvement upon current standard dressings such as Gauze and Neoderm for burn wounds.
水胶体敷料已被开发用于多种伤口愈合类型。特别是，敷料是烧伤成功康复的关键组成部分，烧伤每年导致大量人员不仅遭受身体痛苦，还承受心理和经济痛苦。此外，由于生物相容性，丝素蛋白是组织工程中最安全的材料。在本研究中，我们制备了包含丝素蛋白纳米颗粒的水胶体敷料，以提高水胶体敷料的功效，然后在动物模型中使用这种丝素蛋白纳米颗粒水胶体敷料（SFNHD）治疗烧伤创面。使用拉伸强度和细胞计数试剂盒 - 8检测对SFNHD的结构和性能进行了表征。结果表明水胶体敷料的结构稳定性和细胞生物相容性，表明SFNHD可应用于伤口治疗。为了证明丝素蛋白水胶体敷料治疗烧伤创面的能力，我们将SFNHD与纱布和一种市售敷料Neoderm进行了比较。这项研究清楚地表明，用SFNHD治疗后伤口愈合加速，伤口结构完整性更高，伤口尺寸最小。这些观察结果表明，对于烧伤创面，SFNHD可能是对当前标准敷料如纱布和Neoderm的一种改进。
