Wall M E, Wani M C, Hughes T J, Taylor H
Research Triangle Institute, North Carolina 27709.
J Nat Prod. 1988 Sep-Oct;51(5):866-73. doi: 10.1021/np50059a009.
An antimutagenic assay in Salmonella typhimurium has been utilized for a study of the inhibition of the mutagenic activity of 2-aminoanthracene in the presence of the Ames S-9 metabolic activation preparation by crude organic solvent extracts of plant materials. More than 2000 extracts representing 39 families have been tested to date. Confirmed inhibitory activity has been found in 80 samples. More than 60% were nontoxic. Methods for isolation and characterization of pure compounds are presented. Of particular interest is the utilization of large scale preparative hplc for rapid purification of inhibitory chromatographic fractions that were still highly impure.
利用鼠伤寒沙门氏菌中的抗诱变试验，研究了植物材料的粗有机溶剂提取物在艾姆斯S-9代谢活化制剂存在下对2-氨基蒽诱变活性的抑制作用。迄今为止，已对代表39个科的2000多种提取物进行了测试。在80个样品中发现了确定的抑制活性。超过60%的样品无毒。文中介绍了纯化合物的分离和表征方法。特别值得关注的是利用大规模制备型高效液相色谱法快速纯化仍然高度不纯的抑制性色谱馏分。
