Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu, China.
Chengdu Zoo, Chengdu, China.
Sci Data. 2019 Jan 8;6:180305. doi: 10.1038/sdata.2018.305.
Hog deer (Axis porcinus) is a small deer species in family Cervidae and has been undergoing a serious and global decline during the past decades. Chengdu Zoo currently holds a captive population of hog deer with sufficient genetic diversity in China. We sequenced and de novo assembled its genome sequence in the present study. A total of six different insert-size libraries were sequenced and generated 395 Gb of clean data in total. With aid of the linked reads of 10X Genomics, genome sequence was assembled to 2.72 Gb in length (contig N50, 66.04 Kb; scaffold N50, 20.55 Mb), in which 94.5% of expected genes were detected. We comprehensively annotated 22,473 protein-coding genes, 37,019 tRNAs, and 1,058 Mb repeated sequences. The newly generated reference genome is expected to significantly contribute to comparative analysis of genome biology and evolution within family Cervidae.
豚鹿（Axis porcinus）是鹿科的一种小型鹿种，在过去几十年中经历了严重的全球性衰退。成都动物园目前在中国拥有一个具有足够遗传多样性的豚鹿圈养种群。本研究对其进行了基因组测序和从头组装。总共测序了六个不同的插入大小文库，总共产生了 395GB 的清洁数据。借助 10X Genomics 的连接读取，将基因组序列组装到 2.72GB 的长度（contig N50，66.04 Kb；scaffold N50，20.55Mb），其中检测到 94.5%的预期基因。我们全面注释了 22473 个蛋白质编码基因、37019 个 tRNA 和 1058Mb 重复序列。新生成的参考基因组有望为鹿科内的基因组生物学和进化比较分析做出重大贡献。
