Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases , National Institutes of Health , Bethesda , Maryland 20892 , United States.
J Nat Prod. 2019 Jan 25;82(1):148-153. doi: 10.1021/acs.jnatprod.8b00858. Epub 2019 Jan 9.
A laboratory culture of the colonial marine alga Chrysophaeum taylorii NIES-1699 yielded a set of new bioactive chrysophaentin analogs, and their structures were determined by HRESIMS and NMR spectroscopy. Differences in the metabolites identified between cultured C. taylorii NIES-1699 and field-collected strains from the U.S. Virgin Islands revealed additional structure-activity relationships for the Gram-positive antibiotic activity of the chrysophaentins. The presence of new hemichrysophaentins and a C-C linked biphenyl analog suggest novel features of their biosynthetic pathway. Bayesian analysis of the alignment of the 18S rRNA gene places the microalga C. taylorii in the pelagophyte clade.
实验室培养的海洋藻类 Chrysophaeum taylorii NIES-1699 产生了一组新型生物活性 Chrysophaentin 类似物，并通过 HRESIMS 和 NMR 光谱确定了它们的结构。培养的 C. taylorii NIES-1699 和从美属维尔京群岛采集的野外菌株之间鉴定出的代谢产物的差异揭示了 Chrysophaentins 的革兰氏阳性抗生素活性的其他结构-活性关系。新的半 Chrysophaentins 和 C-C 连接的联苯类似物的存在表明了它们生物合成途径的新特征。对 18S rRNA 基因进行的贝叶斯分析将微藻 C. taylorii 置于 pelagophyte 进化枝中。
