University of Warsaw, Faculty of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.
University of Warsaw, Faculty of Chemistry, Pasteura 1, 02-093 Warsaw, Poland; University of Warsaw, MISMaP College, Banacha 2 C, 02-097 Warsaw, Poland.
Talanta. 2019 Apr 1;195:865-869. doi: 10.1016/j.talanta.2018.12.003. Epub 2018 Dec 6.
A rapid, single-point method for colorimetric creatinine determination has been developed as an alternative for a well-established Jaffé assay, which lacks analytical specificity. 3,5-dinitrobenzoic acid was employed in an alkaline environment as a chromophore instead of picric acid. Apart from the spectrophotometer, a simple optoelectronic detector was applied as an alternative detection method. The conditions of the reaction were optimized to provide satisfactory analytical parameters. The linear ranges of the proposed assays were 40-4000 and 10-300 µmol L of creatinine for conventional spectrophotometry and a PEDD detector, respectively. A broad spectrum of potential interferents was examined. The presence of compounds such as glucose, urea or uric acid was the source of a significantly smaller bias in the examined method than in the case of the Jaffé method variants. The presented method was validated with the use of 10 human urine samples with the creatinine level determined by the recommended method. Two-tail paired Student's t-tests with 9 degrees of freedom at the 95% confidence level showed the agreement between the proposed and the reference methods.
已经开发出一种用于比色法肌酐测定的快速单点方法，作为缺乏分析特异性的既定 Jaffé 测定法的替代方法。3,5-二硝基苯甲酸在碱性环境中用作生色团，而不是苦味酸。除了分光光度计外，还应用了简单的光电探测器作为替代检测方法。优化了反应条件，以提供令人满意的分析参数。建议的测定法的线性范围分别为 40-4000 和 10-300 µmol L 的肌酐，用于常规分光光度法和 PEDD 检测器。检查了潜在干扰物的广泛范围。与 Jaffé 方法变体相比，葡萄糖、尿素或尿酸等化合物的存在对所检查方法的偏差影响要小得多。使用推荐方法测定肌酐水平的 10 个人类尿液样本对所提出的方法进行了验证。95%置信水平下自由度为 9 的双尾配对学生 t 检验表明，所提出的方法与参考方法之间存在一致性。
