Hausmann Christian, Hertz-Kleptow Dominique, Zoschke Christian, Wanjiku Barbara, Wentzien-Odenthal Alexandra, Kerscher Martina, Schäfer-Korting Monika
Institute of Pharmacy, Pharmacology and Toxicology, Freie Universität Berlin, Berlin, Germany.
Institute of Cosmetic Sciences, University of Hamburg, Hamburg, Germany.
Skin Pharmacol Physiol. 2019;32(2):72-80. doi: 10.1159/000495255. Epub 2019 Jan 10.
BACKGROUND/AIMS: The skin provides protection against chemical, physical, and biological stressors, yet the skin morphology changes over the course of life. These changes might affect the skin barrier function and facilitate the onset of age-related diseases. Since orally applied lactic acid bacteria ameliorate signs of aged and atopic skin, we investigated the effects of a topically applied Lactococcus lactis emulsion.
In a blinded, randomized, vehicle-controlled trial, we studied topical Lactococcus effects both in vitro and in 20 healthy female volunteers. Commercially available reconstructed human epidermis (RHE) was treated for 4 days (once daily) and volar forearms were treated for 30 days (twice daily).
Lactococcus formulations improve the skin barrier in RHE as shown by increased filaggrin and human β-defensin-2 expression as well as by the 23% declined mean apparent permeability coefficients for caffeine. A reduction of 18% in transepidermal water loss confirms this effect in humans. Moreover, Lactococcus emulsion optimized skin hydration and surface pH. Skin irritation was not detected.
Lactococcus emulsion improved the skin barrier function with good biocompatibility. Moreover, our study exemplifies the translational predictive capacity of testing on RHE with respect to Lactococcus emulsion.
背景/目的：皮肤可抵御化学、物理和生物应激源，但皮肤形态会随生命进程发生变化。这些变化可能会影响皮肤屏障功能，并促使与年龄相关疾病的发生。由于口服乳酸菌可改善衰老和特应性皮肤的症状，我们研究了局部应用乳酸乳球菌乳液的效果。
在一项双盲、随机、赋形剂对照试验中，我们在体外和20名健康女性志愿者中研究了局部应用乳酸乳球菌的效果。对市售的重组人表皮（RHE）进行4天（每日一次）的处理，对掌侧前臂进行30天（每日两次）的处理。
乳酸乳球菌制剂可改善RHE中的皮肤屏障，表现为丝聚合蛋白和人β-防御素-2表达增加，以及咖啡因的平均表观渗透系数下降23%。经表皮水分流失减少18%证实了其在人体中的这种效果。此外，乳酸乳球菌乳液优化了皮肤水合作用和表面pH值。未检测到皮肤刺激。
乳酸乳球菌乳液改善了皮肤屏障功能，且具有良好的生物相容性。此外，我们的研究例证了在RHE上进行测试对于乳酸乳球菌乳液的转化预测能力。
