Khurelchuluun Ariuntsetseg, Uehara Osamu, Paudel Durga, Morikawa Tetsuro, Kawano Yutaka, Sakata Mashu, Shibata Hiroshi, Yoshida Koki, Sato Jun, Miura Hiroko, Nagayasu Hiroki, Abiko Yoshihiro
Division of Oral Medicine and Pathology, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
Division of Oral and Maxillofacial Surgery, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
Foods. 2021 Jun 4;10(6):1282. doi: 10.3390/foods10061282.
Bee pollen (BP) has a broad range of beneficial effects on health. The aim of this study was to examine the effect of BP on the oral environment, including the microbiome and antimicrobial peptides.
C57BL/6J mice were randomly divided into two groups: control and BP. The BP group was fed with a 5% BP diet for 1 month. Swabs from the oral and buccal mucosa and samples of the intestinal stool were collected. Genomic DNA was extracted and the microbiome was analyzed via 16S rRNA sequencing.
BP inhibited the growth of at a concentration of >2.5%. The metagenomic study showed that the abundance of genus was significantly elevated in the oral and intestinal microbiomes of the BP group when compared to those of the control group. Significant alterations in alpha and beta diversity were observed between the oral microbiomes of the two groups. The mRNA levels of beta-defensin-2 and -3 were significantly upregulated in the buccal mucosa of the BP group.
A BP diet may have a beneficial effect on oral and systemic health by modulating the bacterial flora and antimicrobial peptides of the oral cavity. Further investigations are needed to clarify how a BP diet affects overall human health.
蜂花粉（BP）对健康具有广泛的有益作用。本研究的目的是研究BP对口腔环境的影响，包括微生物群和抗菌肽。
将C57BL/6J小鼠随机分为两组：对照组和BP组。BP组喂食含5%BP的饮食1个月。采集口腔和颊黏膜拭子以及肠道粪便样本。提取基因组DNA，并通过16S rRNA测序分析微生物群。
BP在浓度>2.5%时抑制了[具体细菌名称未给出]的生长。宏基因组研究表明，与对照组相比，BP组口腔和肠道微生物群中[具体菌属名称未给出]的丰度显著升高。两组口腔微生物群之间观察到α和β多样性的显著变化。BP组颊黏膜中β-防御素-2和-3的mRNA水平显著上调。
BP饮食可能通过调节口腔细菌菌群和抗菌肽对口腔和全身健康产生有益影响。需要进一步研究以阐明BP饮食如何影响人类整体健康。
