Wongta Anurak, Sawarng Nootchakarn, Tongchai Phannika, Sutan Kunrunya, Kerdnoi Tanyaporn, Prapamontol Tippawan, Hongsibsong Surat
NCD Center of Excellence, Environment and Health Research Unit, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
Environmental Science Ph.D. Program, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
J Toxicol. 2018 Nov 29;2018:4168034. doi: 10.1155/2018/4168034. eCollection 2018.
Biomarkers of pesticide exposure are generally lacking in communities where agricultural crops are grown. The purpose of this study was to focus on evaluating biomarkers of pesticide exposure in people living in an agricultural area of San Pa Tong District in Chiang Mai Province, northern Thailand.
One hundred and twenty-four participants (38 nonfarm workers, 38 rice growers, 31 longan growers, and 17 vegetable growers) from San Pa Tong District gave consent to participate in the study. Pesticide exposure was assessed by determining acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) levels in blood samples using Ellman's method and measuring 6-dialkylphosphate metabolites (DAPs), 3-phenoxybenzoic acid (3-PBA), and glyphosate in urine samples using chromatographic methods.
AChE and BChE activities in the nonfarm worker group had higher level than those in the grower groups. DAPs were detected in almost all urine samples and 3-PBA was detected in 12-45% of each group, while glyphosate was found in 11 - 30% among the three groups of growers but not in nonfarm workers.
In this study, participants living in an agricultural area of San Pa Tong District were exposed to organophosphate, synthetic pyrethroid, and glyphosate through multiple pathways.
在种植农作物的社区，通常缺乏农药暴露的生物标志物。本研究的目的是着重评估泰国北部清迈府桑帕通区农业地区居民的农药暴露生物标志物。
来自桑帕通区的124名参与者（38名非农业工人、38名水稻种植者、31名龙眼种植者和17名蔬菜种植者）同意参与本研究。通过使用埃尔曼方法测定血样中的乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）水平，并使用色谱方法测量尿样中的6-二烷基磷酸酯代谢物（DAPs）、3-苯氧基苯甲酸（3-PBA）和草甘膦来评估农药暴露情况。
非农业工人群体中的AChE和BChE活性水平高于种植者群体。几乎所有尿样中都检测到了DAPs，每组中有12%-45%的样本检测到了3-PBA，而在三组种植者中，有11%-30%的样本检测到了草甘膦，非农业工人中未检测到。
在本研究中，居住在桑帕通区农业地区的参与者通过多种途径接触有机磷、拟除虫菊酯和草甘膦。
