Department of Chemistry, University of Oxford, South Parks Road, Oxford, OX1 3QR, UK.
Manchester Institute of Biotechnology, School of Chemistry, University of Manchester, Manchester, M1 7DN, UK.
Angew Chem Int Ed Engl. 2019 Apr 1;58(15):4948-4952. doi: 10.1002/anie.201814370. Epub 2019 Feb 14.
In living cells, redox chains rely on nanoconfinement using tiny enclosures, such as the mitochondrial matrix or chloroplast stroma, to concentrate enzymes and limit distances that nicotinamide cofactors and other metabolites must diffuse. In a chemical analogue exploiting this principle, nicotinamide adenine dinucleotide phosphate (NADPH) and NADP are cycled rapidly between ferredoxin-NADP reductase and a second enzyme-the pairs being juxtaposed within the 5-100 nm scale pores of an indium tin oxide electrode. The resulting electrode material, denoted (FNR+E2)@ITO/support, can drive and exploit a potentially large number of enzyme-catalysed reactions.
在活细胞中，氧化还原链依赖于纳米限制，使用微小的封闭物，如线粒体基质或叶绿体基质，来浓缩酶并限制烟酰胺辅酶和其他代谢物必须扩散的距离。在利用这一原理的化学类似物中，烟酰胺腺嘌呤二核苷酸磷酸（NADPH）和 NADP 快速地在铁氧还蛋白-NADP 还原酶和第二种酶之间循环，这两种酶在氧化铟锡电极的 5-100nm 尺度孔内并列。所得的电极材料，记为（FNR+E2）@ITO/support，能够驱动和利用大量的酶催化反应。
