Cardoso Susana M, Falcão Soraia I, Peres António M, Domingues Maria R M
CERNAS - Escola Superior Agrária, Instituto Politécnico de Coimbra, Bencanta, 3040-316 Coimbra, Portugal; CIMO - Escola Superior Agrária, Instituto Politécnico de Bragança, Campus de Sta. Apolónia, 5301-855 Bragança, Portugal.
CIMO - Escola Superior Agrária, Instituto Politécnico de Bragança, Campus de Sta. Apolónia, 5301-855 Bragança, Portugal.
Food Chem. 2011 Nov 15;129(2):291-296. doi: 10.1016/j.foodchem.2011.04.049. Epub 2011 Apr 24.
Olive mill wastewaters (OMW) are a potential source of biophenols, but they have a complex composition with many unknown phenolics. The analysis of purified methanol extracts from two Portuguese OMW by electrospray mass spectrometry in the negative mode showed [M-H] ions at m/z 539 and m/z 523, corresponding respectively to oleuropein and ligstroside isomers which contain the glucose unit linked to its aromatic moiety. Also, the fragmentation pathway of the [M-H] ions at m/z 863, 685 and 847 indicated the presence of a diglucoside derivative of the oleuropein isomer and of mono- and diglucosides of the ligstroside isomer, respectively. Moreover, the two OMW samples contained an elenoic derivative of the ion at m/z 685 and a degradation product (m/z 453) of the [M-H] ion at m/z 523. Future studies focusing on the abundance of these compounds on OMW, as well as their bioactivities, will determine their possible industrial exploitation.
橄榄榨油厂废水（OMW）是生物酚的潜在来源，但它们的成分复杂，含有许多未知的酚类物质。通过电喷雾质谱在负离子模式下对两种葡萄牙OMW的纯化甲醇提取物进行分析，结果显示在m/z 539和m/z 523处出现[M-H]离子，分别对应于橄榄苦苷和裂环烯醚萜苷异构体，它们含有与芳香部分相连的葡萄糖单元。此外，m/z 863、685和847处的[M-H]离子的碎裂途径分别表明存在橄榄苦苷异构体的二糖苷衍生物以及裂环烯醚萜苷异构体的单糖苷和二糖苷。此外，两个OMW样品中含有m/z 685处离子的elenoic衍生物以及m/z 523处[M-H]离子的降解产物（m/z 453）。未来针对这些化合物在OMW中的丰度及其生物活性的研究，将确定它们可能的工业开发价值。
