Instituto Multidisciplinario de Biología Vegetal (IMBIV-CONICET), Universidad Nacional de Córdoba, POB 495, Córdoba 5000, Argentina.
Department of Botany and Biodiversity Research, Faculty of Life Sciences, University of Vienna, Rennweg 14, 1030 Vienna, Austria.
Molecules. 2019 Jan 9;24(2):216. doi: 10.3390/molecules24020216.
Plastid genome analysis of non-model organisms provides valuable information for basic research e.g., molecular evolutionary genomics, phylogeny and phylogeography. is the most widespread species of the genus and it is a common grass that is found across Eurasia and North America. Scattered populations in regions of appropriate ecological conditions are also found in Australia, New Zealand and southern South America, where it is sympatric with . We analyzed the plastid genome of a sample of of the Austrian Alps using high-throughput sequencing. The plastid (cp) genome shows the typical quadripartite structure with a length of 135,340 bp, comprising a large single-copy (LSC) region of 79,992 bp, a small single-copy (SSC) region of 12,572 bp and two inverted repeats (IR) regions of 21,388 bp each. It contains 115 genes, including 85 protein-coding genes, four ribosomal RNA genes and 30 transfer RNA genes. The GC content (%), number of repeats and microsatellites, RNA editing sites and codon usage were highly similar to those of . The results of this present study highlight the extremely conserved nature of the cp genome in this group, since the comparison involved individuals separated by about 13,000 km, from the Alps to Antarctica.
非模式生物的质体基因组分析为基础研究提供了有价值的信息，例如分子进化基因组学、系统发育和系统地理学。是该属中分布最广的物种，是一种常见的草，分布在欧亚大陆和北美洲。在澳大利亚、新西兰和南美洲南部的适宜生态条件地区也有分散的种群，在那里它与共存。我们使用高通量测序分析了奥地利阿尔卑斯山的一个样本的质体基因组。质体（cp）基因组显示出典型的四分体结构，长度为 135340bp，包括一个长的单一拷贝（LSC）区域，长 79992bp，一个小的单一拷贝（SSC）区域，长 12572bp 和两个反向重复（IR）区域，每个区域长 21388bp。它包含 115 个基因，包括 85 个蛋白质编码基因、4 个核糖体 RNA 基因和 30 个转移 RNA 基因。GC 含量（%）、重复和微卫星的数量、RNA 编辑位点和密码子使用与相似。本研究的结果突出了该组质体基因组的高度保守性质，因为比较涉及到的个体相隔约 13000 公里，从阿尔卑斯山到南极洲。
