Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov St., 119991 Moscow, Russia.
Genes (Basel). 2022 Apr 26;13(5):762. doi: 10.3390/genes13050762.
Subpolar and polar ecotypes of (Popl.) Roshev, (L.) P. Beauv, and E. Desv. are well adapted to stressful environmental conditions, which make them useful model plants for genetic research and breeding. For the first time, the comparative repeatome analyses of subpolar and polar , , and was performed using RepeatExplorer/TAREAN pipelines and FISH-based chromosomal mapping of the identified satellite DNA families (satDNAs). In the studied species, mobile genetic elements of class 1 made up the majority of their repetitive DNA; interspecific variations in the total amount of Ty3/Gypsy and Ty1/Copia retroelements, DNA transposons, ribosomal, and satellite DNA were revealed; 12-18 high confident and 7-9 low confident putative satDNAs were identified. According to BLAST, most satDNAs demonstrated sequence similarity with satDNAs of and indicating their common origin. Chromosomal mapping of 45S rDNA, 5S rDNA, and satDNAs of allowed us to construct the species karyograms and detect new molecular chromosome markers important for species. Our findings confirmed that genomes of and were more closely related compared to according to repeatome composition and patterns of satDNA chromosomal distribution.
亚极区和极地生态型的 (Popl.)Roshev,(L.)P. Beauv.和 E. Desv. 对胁迫环境条件具有很好的适应性,使它们成为遗传研究和育种的有用模式植物。首次使用 RepeatExplorer/TAREAN 管道和基于 FISH 的鉴定卫星 DNA 家族(satDNAs)的染色体作图对亚极区和极地 、 、 进行了比较重复组分析。在所研究的物种中,1 类移动遗传元件构成了它们重复 DNA 的大部分;揭示了 Ty3/Gypsy 和 Ty1/Copia 反转录转座子、DNA 转座子、核糖体和卫星 DNA 的总量的种间变异;鉴定了 12-18 个高置信和 7-9 个低置信的假定 satDNAs。根据 BLAST,大多数 satDNAs 与 和 的 satDNAs 具有序列相似性,表明它们具有共同的起源。45S rDNA、5S rDNA 和 satDNAs 的染色体作图使我们能够构建物种核型,并检测到对 物种重要的新分子染色体标记。我们的研究结果证实,根据重复组组成和 satDNA 染色体分布模式,与 相比, 和 的基因组更为密切相关。