Department of Oral Maxillofacial Surgery, Division of Regenerative Orofacial Medicine, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.
Department of Oral Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf; 20246 Hamburg, Germany.
Int J Mol Sci. 2019 Jan 10;20(2):255. doi: 10.3390/ijms20020255.
Magnesium (Mg)-based biomaterials are promising candidates for bone and tissue regeneration. Alloying and surface modifications provide effective strategies for optimizing and tailoring their degradation kinetics. Nevertheless, biocompatibility analyses of Mg-based materials are challenging due to its special degradation mechanism with continuous hydrogen release. In this context, the hydrogen release and the related (micro-) milieu conditions pretend to strictly follow in vitro standards based on ISO 10993-5/-12. Thus, special adaptions for the testing of Mg materials are necessary, which have been described in a previous study from our group. Based on these adaptions, further developments of a test procedure allowing rapid and effective in vitro cytocompatibility analyses of Mg-based materials based on ISO 10993-5/-12 are necessary. The following study introduces a new two-step test scheme for rapid and effective testing of Mg. Specimens with different surface characteristics were produced by means of plasma electrolytic oxidation (PEO) using silicate-based and phosphate-based electrolytes. The test samples were evaluated for corrosion behavior, cytocompatibility and their mechanical and osteogenic properties. Thereby, two PEO ceramics could be identified for further in vivo evaluations.
镁（Mg）基生物材料是骨和组织再生的有前途的候选材料。合金化和表面改性提供了优化和定制其降解动力学的有效策略。然而，由于其特殊的降解机制伴随着持续的氢释放，Mg 基材料的生物相容性分析具有挑战性。在这种情况下，氢的释放及其相关的（微观）环境条件应该严格遵循基于 ISO 10993-5/-12 的体外标准。因此，需要对 Mg 材料的测试进行特殊的适应，我们小组之前的一项研究中已经对此进行了描述。基于这些适应，需要进一步开发一种测试程序，以便基于 ISO 10993-5/-12 快速有效地进行 Mg 基材料的体外细胞相容性分析。以下研究介绍了一种新的两步测试方案，用于快速有效地测试 Mg。通过使用硅酸盐基和磷酸盐基电解质的等离子体电解氧化（PEO）方法生产具有不同表面特性的试样。对测试样品的腐蚀行为、细胞相容性以及机械和成骨性能进行了评估。由此，可以确定两种 PEO 陶瓷材料可进一步进行体内评估。
