Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Cell Physiol. 2019 Aug;234(8):14234-14245. doi: 10.1002/jcp.28119. Epub 2019 Jan 13.
Rn7SK is a conserved small nuclear noncoding RNA which its function in aging has not been studied. Recently, we have demonstrated that Rn7SK overexpression reduces cell viability and is significantly downregulated in stem cells, human tumor tissues, and cell lines. In this study, we analyzed the role of Rn7SK on senescence in adipose tissue-derived mesenchymal stem cells (AD-MSCs). For this purpose, Rn7SK expression was downregulated and upregulated via transfection and transduction, respectively, in AD-MSCs and subsequently, various distinct characteristics of senescence including cell viability, proliferation, colony formation, senescence-associated β galactosidase activity, and differentiation potency was analyzed. Our results demonstrated the transient knockdown of Rn7SK in MSCs leads to delayed senescence, while its overexpressions shows opposite effects. When osteogenic differentiation was started, however, they exhibited a greater differentiation potential than the original MSCs, suggesting a potential tool for stem cell-based regenerative medicine.
Rn7SK 是一种保守的小核非编码 RNA，其在衰老中的功能尚未得到研究。最近，我们已经证明 Rn7SK 的过表达会降低细胞活力，并且在干细胞、人类肿瘤组织和细胞系中显著下调。在这项研究中，我们分析了 Rn7SK 对脂肪组织来源的间充质干细胞 (AD-MSCs) 衰老的作用。为此，我们通过转染和转导分别下调和上调 AD-MSCs 中的 Rn7SK 表达，然后分析衰老的各种不同特征，包括细胞活力、增殖、集落形成、衰老相关β半乳糖苷酶活性和分化潜能。我们的结果表明，MSCs 中 Rn7SK 的瞬时敲低导致衰老延迟，而过表达则产生相反的效果。然而，当开始成骨分化时，它们表现出比原始 MSCs 更高的分化潜能，这表明它可能成为基于干细胞的再生医学的一种潜在工具。
