Faculté des Sciences Pharmaceutiques, Unité de Recherche Œnologie, EA 4577, USC 1366 INRA, Equipe Molécules d'Intérêt Biologique - ISVV, Univ. Bordeaux, 33882, Villenave d'Ornon cedex, France.
Phytochem Anal. 2019 May;30(3):320-331. doi: 10.1002/pca.2815. Epub 2019 Jan 15.
Grapevine wood and roots are by-products obtained during vineyard management. This plentiful biomass is known to be rich in stilbenes and can be used as a source of high-value compounds as well as active natural extracts. However, the stilbenes in grapevine wood and roots from different cultivars and rootstocks remain to be characterized.
The present study investigated the stilbene content of eight major Vitis vinifera cultivars and six different rootstocks. In addition, the distribution of stilbenes was established for each of seven parts into which the plants were sub-divided.
For stilbene characterization and quantification purposes, an ultra-high performance liquid chromatography-diode array detector-mass spectrometry (UHPLC-DAD-MS/MS) analysis of different samples was carried out. Moreover, structural data of stilbenes was unambiguously studied by nuclear magnetic resonance (NMR) spectra.
Whatever the cultivar/rootstock combination, stilbenes were found to be oligomerized from the aerial part to the root system. Furthermore, stilbene content varied widely depending on the cultivars and rootstocks. For instance, the cultivars Merlot, Tannat and Gamay noir were the richest in stilbenes while the rootstocks Gravesac, Fercal and 3390C contained the highest amounts.
These findings provide insight into the knowledge that major grapevine cultivars and rootstocks can be used as a potential source of complex stilbenes.
葡萄藤木和根系是葡萄园管理过程中的副产品。这种丰富的生物质已知富含白藜芦醇，可用作高价值化合物的来源以及活性天然提取物。然而，不同品种和砧木的葡萄藤木和根系中的白藜芦醇仍有待表征。
本研究调查了 8 个主要的酿酒葡萄品种和 6 个不同砧木的白藜芦醇含量。此外，还对植物分为 7 个部分的每个部分中的白藜芦醇分布进行了建立。
为了进行白藜芦醇的特征和定量分析，对不同样品进行了超高效液相色谱-二极管阵列检测器-质谱（UHPLC-DAD-MS/MS）分析。此外，通过核磁共振（NMR）谱对白藜芦醇的结构数据进行了明确研究。
无论品种/砧木组合如何，白藜芦醇都从地上部分到根系发生了聚合。此外，白藜芦醇含量因品种和砧木而异。例如，梅洛、塔那和佳美娜等品种的白藜芦醇含量最丰富，而 Gravesac、Fercal 和 3390C 等砧木的含量最高。
这些发现深入了解了主要的酿酒葡萄品种和砧木可以作为复杂白藜芦醇的潜在来源的知识。
