Department of Chemistry and Biochemistry , University of Oklahoma , Norman , Oklahoma 73019 , United States.
Anal Chem. 2019 Feb 5;91(3):1738-1742. doi: 10.1021/acs.analchem.8b05774. Epub 2019 Jan 18.
Existing single cell mass spectrometry (SCMS) sampling platforms are largely designed to work only with immobilized cells and not the suspended cells isolated from patient samples. Here, we present a novel method that integrates a commercially available cell manipulation system commonly used for in vitro fertilization with the Single-probe SCMS sampling technology. The combined Single-probe SCMS/cell manipulating platform is capable of rapidly analyzing intracellular species in real time from a suspension leukemia cell line. A broad range of molecular species was detected, and species of interest were verified using tandem MS (MS/MS). Experimental results were analyzed utilizing statistical analyses such as principle component analysis (PCA) and t-tests. The developed SCMS/cell manipulation system is a versatile tool to provide rapid single cell analysis of broad types of patient cell samples.
现有的单细胞质谱（SCMS）采样平台在很大程度上仅设计用于固定细胞，而不是从患者样本中分离的悬浮细胞。在这里，我们提出了一种新方法，该方法将商业上可用于体外受精的细胞操作系统与单探针 SCMS 采样技术相结合。组合的单探针 SCMS/细胞操作平台能够从悬浮白血病细胞系中实时快速分析细胞内物质。检测到广泛的分子物质，并使用串联质谱（MS/MS）对感兴趣的物质进行了验证。利用主成分分析（PCA）和 t 检验等统计分析方法对实验结果进行了分析。开发的 SCMS/细胞操作系统是一种通用工具，可用于快速分析广泛类型的患者细胞样本的单细胞。
