Taylor P B, Burd E M, Tabbara K F
Geisinger Medical Center, Department of Ophthalmology, Danville, Pennsylvania.
Int Ophthalmol. 1988;12(1):81-6. doi: 10.1007/BF00133786.
Various techniques which use monoclonal antibodies to detect Chlamydia trachomatis in clinical specimens are reviewed. An investigation comparing the efficacy of immunofluorescent staining with Giemsa staining in detecting Chlamydia in conjunctival scrapings from cases of active trachoma is presented. Sixty-two eyes of schoolboys with moderate to severe trachoma were studied. Giemsa staining detected chlamydial inclusion bodies in 34 percent of the specimens. Free elementary bodies were detected by fluorescent monoclonal antibody in 21 percent. Eleven percent were positive by both Giemsa and immunofluorescence and 55 percent were positive by either Giemsa and/or immunofluorescence. The addition of fluorescent monoclonal antibody assay to routine Giemsa staining resulted in an increase in the yield of positive specimens by 29 percent.
本文综述了多种利用单克隆抗体检测临床标本中沙眼衣原体的技术。文中呈现了一项关于比较免疫荧光染色与吉姆萨染色在检测活动性沙眼患者结膜刮片中衣原体效力的调查。研究对象为62名患有中度至重度沙眼的男学生的眼睛。吉姆萨染色在34%的标本中检测到衣原体包涵体。荧光单克隆抗体检测到21%的游离原体。11%的标本吉姆萨染色和免疫荧光均呈阳性，55%的标本吉姆萨染色和/或免疫荧光呈阳性。在常规吉姆萨染色中添加荧光单克隆抗体检测，使阳性标本的检出率提高了29%。
