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免疫荧光法与吉姆萨染色法对环己酰亚胺处理的 McCoy 细胞中沙眼衣原体包涵体染色敏感性的比较。

A comparison of the sensitivity of immunofluorescence and Giemsa for staining Chlamydia trachomatis inclusions in cycloheximide-treated McCoy cells.

作者信息

Munday P E, Johnson A P, Thomas B J, Taylor-Robinson D

出版信息

J Clin Pathol. 1980 Feb;33(2):177-9. doi: 10.1136/jcp.33.2.177.

DOI:10.1136/jcp.33.2.177
PMID:6988463
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1146016/
Abstract

In a clinical study of 190 men with non-gonococcal urethritis, Chlamydia trachomatis inclusions were sought in cycloheximide-treated McCoy cells by an indirect immunofluorescent antibody technique. The method was consistently reliable over a period of two years, and the results were obtained within 24 hours of a patient's attendance. The results correlated with those obtained by Giemsa staining in 91.6% of patients, and the new method was at least as sensitive as the established Giemsa-staining method.

摘要

在一项针对190名非淋菌性尿道炎男性患者的临床研究中，采用间接免疫荧光抗体技术，在经放线菌酮处理的 McCoy 细胞中寻找沙眼衣原体包涵体。该方法在两年时间里始终可靠，且能在患者就诊后24小时内得出结果。在91.6%的患者中，该方法的结果与吉姆萨染色法的结果相关，并且新方法至少与既定的吉姆萨染色法一样灵敏。

相似文献

1
A comparison of the sensitivity of immunofluorescence and Giemsa for staining Chlamydia trachomatis inclusions in cycloheximide-treated McCoy cells.免疫荧光法与吉姆萨染色法对环己酰亚胺处理的 McCoy 细胞中沙眼衣原体包涵体染色敏感性的比较。
J Clin Pathol. 1980 Feb;33(2):177-9. doi: 10.1136/jcp.33.2.177.
2
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3
[Prostato-urethritis due to chlamydia].
Z Hautkr. 1982 Jun 1;57(11):790-802.
4
Early detection of chlamydial inclusions combining the use of cycloheximide-treated McCoy cells and immunofluorescence staining.结合使用环己酰亚胺处理的 McCoy 细胞和免疫荧光染色法对衣原体包涵体进行早期检测。
J Clin Microbiol. 1977 Sep;6(3):285-92. doi: 10.1128/jcm.6.3.285-292.1977.
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[Comparative evaluation of various direct and culture methods for the diagnosis of genital Chlamydia trachomatis infections].
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引用本文的文献

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Maedica (Bucur). 2018 Jun;13(2):131-136. doi: 10.26574/maedica.2018.13.2.131.
2
Chlamydia trachomatis in general practice: a preliminary report.全科医疗中的沙眼衣原体：初步报告。
J R Coll Gen Pract. 1982 Sep;32(242):562-3.
3
Clinical and microbiological study of non-gonococcal urethritis with particular reference to non-chlamydial disease.非淋菌性尿道炎的临床与微生物学研究，特别涉及非衣原体疾病。
Br J Vener Dis. 1981 Oct;57(5):327-33. doi: 10.1136/sti.57.5.327.
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Prevalence of chlamydial infection in promiscuous women.滥交女性衣原体感染的患病率。
Br J Vener Dis. 1983 Apr;59(2):103-4. doi: 10.1136/sti.59.2.103.
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Is one swab enough to detect chlamydial infection of the cervix?一支拭子足以检测宫颈衣原体感染吗？
Br J Vener Dis. 1984 Dec;60(6):384-6. doi: 10.1136/sti.60.6.384.
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Chlamydial proctitis?衣原体性直肠炎？
Genitourin Med. 1985 Dec;61(6):376-8. doi: 10.1136/sti.61.6.376.
7
Evaluation of single whole inclusion serum test for IgG antibody to Chlamydia trachomatis in asymptomatic women.无症状女性沙眼衣原体IgG抗体单一全血包涵体血清检测的评估
Genitourin Med. 1987 Feb;63(1):22-5. doi: 10.1136/sti.63.1.22.
8
Comparison of a fluorescent monoclonal antibody assay and a tissue culture assay for routine detection of infections caused by Chlamydia trachomatis.
Eur J Clin Microbiol. 1986 Oct;5(5):554-8. doi: 10.1007/BF02017704.
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Monoclonal antibodies in the laboratory diagnosis of trachoma.单克隆抗体在沙眼实验室诊断中的应用
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本文引用的文献

1
Cultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells.沙眼衣原体在经放线菌酮处理的 McCoy 细胞中的培养。
J Clin Microbiol. 1977 Oct;6(4):328-31. doi: 10.1128/jcm.6.4.328-331.1977.
2
Comparison of various McCoy cell treatment procedures used for detection of Chlamydia trachomatis.用于检测沙眼衣原体的各种 McCoy 细胞处理程序的比较。
J Clin Microbiol. 1979 Aug;10(2):198-201. doi: 10.1128/jcm.10.2.198-201.1979.
3
Early detection of chlamydial inclusions combining the use of cycloheximide-treated McCoy cells and immunofluorescence staining.结合使用环己酰亚胺处理的 McCoy 细胞和免疫荧光染色法对衣原体包涵体进行早期检测。
J Clin Microbiol. 1977 Sep;6(3):285-92. doi: 10.1128/jcm.6.3.285-292.1977.