免疫荧光法与吉姆萨染色法对环己酰亚胺处理的 McCoy 细胞中沙眼衣原体包涵体染色敏感性的比较。
A comparison of the sensitivity of immunofluorescence and Giemsa for staining Chlamydia trachomatis inclusions in cycloheximide-treated McCoy cells.
作者信息
Munday P E, Johnson A P, Thomas B J, Taylor-Robinson D
出版信息
J Clin Pathol. 1980 Feb;33(2):177-9. doi: 10.1136/jcp.33.2.177.
Abstract
In a clinical study of 190 men with non-gonococcal urethritis, Chlamydia trachomatis inclusions were sought in cycloheximide-treated McCoy cells by an indirect immunofluorescent antibody technique. The method was consistently reliable over a period of two years, and the results were obtained within 24 hours of a patient's attendance. The results correlated with those obtained by Giemsa staining in 91.6% of patients, and the new method was at least as sensitive as the established Giemsa-staining method.
摘要
在一项针对190名非淋菌性尿道炎男性患者的临床研究中，采用间接免疫荧光抗体技术，在经放线菌酮处理的 McCoy 细胞中寻找沙眼衣原体包涵体。该方法在两年时间里始终可靠，且能在患者就诊后24小时内得出结果。在91.6%的患者中，该方法的结果与吉姆萨染色法的结果相关，并且新方法至少与既定的吉姆萨染色法一样灵敏。
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本文引用的文献
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J Clin Microbiol. 1977 Oct;6(4):328-31. doi: 10.1128/jcm.6.4.328-331.1977.
2
Comparison of various McCoy cell treatment procedures used for detection of Chlamydia trachomatis.用于检测沙眼衣原体的各种 McCoy 细胞处理程序的比较。
J Clin Microbiol. 1979 Aug;10(2):198-201. doi: 10.1128/jcm.10.2.198-201.1979.
3
Early detection of chlamydial inclusions combining the use of cycloheximide-treated McCoy cells and immunofluorescence staining.结合使用环己酰亚胺处理的 McCoy 细胞和免疫荧光染色法对衣原体包涵体进行早期检测。
J Clin Microbiol. 1977 Sep;6(3):285-92. doi: 10.1128/jcm.6.3.285-292.1977.
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