Darougar S, Woodland R M, Jones B R, Houshmand A, Farahmandian H A
Br J Ophthalmol. 1980 Apr;64(4):276-8. doi: 10.1136/bjo.64.4.276.
The sensitivity of an indirect fluorescent antibody (FA) staining technique for detecting chlamydial inclusions in scrapings from the whole conjunctiva (upper tarsus, upper fornix, and lower lid) was compared with the sensitivity of culture in irradiated McCoy cells for the diagnosis of hyperendemic trachoma. In a group of 211 patients with various grades of active trachoma from the Bandar Abbas area of Southern Iran 42 patients were positive for chlamydiae by either method. There was little difference between the rates of positivity of FA staining of the scrapings from the whole conjunctiva (28 positives) and culture in irradiated McCoy cells (32 positives). In the patients included in this study chlamydial inclusions were detected in 15 eyes by examination of FA stained scrapings taken from the upper tarsal conjunctiva, whereas inclusions were detected in 40 eyes by the additional examination of scrapings taken from the upper fornix and lower lid (P less than 0.001). The examination of FA stained scrapings taken from the whole conjunctiva and spread as a single but larger smear may provide a satisfactory alternative to cell culture methods for the diagnosis of trachoma, particularly for field studies when cell culture facilities are not available.
将检测全结膜（上睑板、上穹窿和下睑）刮片中衣原体包涵体的间接荧光抗体（FA）染色技术的敏感性，与在经辐射的 McCoy 细胞中培养用于诊断高度流行沙眼的敏感性进行了比较。在来自伊朗南部阿巴斯港地区的 211 例不同程度活动性沙眼患者中，两种方法检测出衣原体阳性的患者有 42 例。全结膜刮片的 FA 染色阳性率（28 例阳性）与经辐射的 McCoy 细胞培养阳性率（32 例阳性）之间差异不大。在本研究纳入的患者中，通过检查取自上睑结膜的 FA 染色刮片，在 15 只眼中检测到衣原体包涵体，而通过额外检查取自上穹窿和下睑的刮片，在 40 只眼中检测到包涵体（P＜0.001）。检查取自全结膜并作为单个但更大涂片铺展的 FA 染色刮片，可能为沙眼诊断提供一种令人满意的替代细胞培养方法，特别是在没有细胞培养设施的现场研究中。
