National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University, Chongqing 400038, PR China.
Chongqing Technical Center for Drug Evaluation & Certification, Chongqing 400014, PR China.
Vaccine. 2019 Feb 14;37(8):1053-1061. doi: 10.1016/j.vaccine.2018.12.066. Epub 2019 Jan 18.
Some plant polysaccharides (PPSs) had been used as the adjuvants for systemic vaccination. In this study, we investigated whether PPSs could exhibit adjuvant effect at the mucosa. Groups of mice were intranasally immunized with Epimedium Polysaccharide (EPS), Trollius chinensis polysaccharide (TCPS), Siberian solomonseal rhizome polysaccharide (SSRPS) and Astragalus polysaccharides (APS) together with ovalbumin (OVA). Significantly higher levels of OVA-specific IgG in serum and secretory IgA in saliva, vaginal wash and intestinal lavage fluid were induced after immunization with OVA plus one of the four PPSs compared to OVA alone. Antigen absorption and TLR2 (Toll-like receptor 2) activation may be related to their mucosal adjuvant effect. Of note, when APS used as an adjuvant, intranasally vaccination with recombination UreB (rUreB, Urease subunit B) conferred more robust protection against Helicobacter pylori (H. pylori). Immunized with rUreB in combination APS resulted in mixed specific Th1 and Th17 immune response, which may contribute to the inhibition of H. pylori colonization. Though specific Th2-dominant responses were elicited when the other three PPS intranasally immunized with rUreB, no significant difference in the protective effect were found between those groups and rUreb alone group. Taken together, the four PPSs may be promising candidates for mucosal adjuvant, and APS could enhance rUreB-specific protective immunity against H. pylori infection.
一些植物多糖（PPS）已被用作全身疫苗的佐剂。在这项研究中，我们研究了 PPS 是否可以在粘膜中表现出佐剂作用。将各组小鼠用淫羊藿多糖（EPS）、金莲花多糖（TCPS）、西伯利亚麦冬根多糖（SSRPS）和黄芪多糖（APS）与卵清蛋白（OVA）一起经鼻腔免疫。与单独用 OVA 免疫相比，用 OVA 加四种 PPS 之一免疫后，血清中 OVA 特异性 IgG 水平和唾液、阴道冲洗液和肠灌洗液中的分泌型 IgA 水平显著升高。抗原吸收和 TLR2（Toll 样受体 2）激活可能与其粘膜佐剂作用有关。值得注意的是，当 APS 用作佐剂时，鼻腔内接种重组 UreB（rUreB，尿素酶亚基 B）可更有效地预防幽门螺杆菌（H. pylori）感染。用 rUreB 联合 APS 免疫可产生混合的特异性 Th1 和 Th17 免疫反应，这可能有助于抑制 H. pylori 的定植。虽然用另外三种 PPS 鼻腔内免疫 rUreB 时会引起特异性 Th2 优势反应，但在保护性效果方面，这些组与 rUreb 单独组之间没有发现显著差异。总之，这四种 PPS 可能是有前途的粘膜佐剂候选物，而 APS 可以增强 rUreB 特异性对 H. pylori 感染的保护性免疫。
