Dinakaran Vasudevan, Mandape Sammed N, Shuba Kristina, Pratap Siddharth, Sakhare Shruti S, Tabatabai Mohammad Ali, Smoot Duane T, Farmer-Dixon Cherae M, Kesavalu Lakshmyya N, Adunyah Samuel Evans, Southerland Janet Hayes, Gangula Pandu R
Department of ODS & Research, School of Dentistry, Meharry Medical College, Nashville, TN, United States.
Bioinformatics Core, School of Graduate Studies/Research & School of Medicine, Meharry Medical College, Nashville, TN, United States.
Front Microbiol. 2019 Jan 4;9:3220. doi: 10.3389/fmicb.2018.03220. eCollection 2018.
Impaired colon motility is one of the leading problems associated with inflammatory bowel disease (IBD). An expanding body of evidence supports the role of microbiome in normal gut function and in progression of IBD. The objective of this work is to determine whether diseased full thickness colon specimens, including the neuromuscular region (critical for colon motility function), contain specific oral and gut pathogens. In addition, we compared the differences in colon microbiome between Caucasians (CA) and African Americans (AA). Thirty-nine human full thickness colon (diseased colon and adjacent healthy colon) specimens were collected from Crohn's Colitis (CC) or Ulcerative Colitis (UC) patients while they underwent elective colon surgeries. We isolated and analyzed bacterial ribosomal RNA (rRNA) from colon specimens by amplicon sequencing of the 16S rRNA gene region. The microbiome proportions were quantified into Operational Taxonomic Units (OTUs) by analysis with Quantitative Insights Into Microbial ecology (QIIME) platform. Two hundred twenty-eight different bacterial species were identified by QIIME analysis. However, we could only decipher the species name of fifty-three bacteria. Our results show that proportion of non-detrimental bacteria in CC or UC colon samples were altered compared to adjacent healthy colon specimens. We further show, for the first time in full thickness colon specimens, that microbiome of CC and UC diseased specimens is dominated by putative oral pathogens belonging to the Phyla Firmicutes (), and Fusobacteria (). In addition, we have identified patterns of differences in microbiome levels between CA and AA specimens with potential implications for health disparities research. Overall, our results suggest a significant association between oral and gut microbes in the modulation of colon motility in colitis patients.
结肠动力受损是炎症性肠病（IBD）相关的主要问题之一。越来越多的证据支持微生物群在正常肠道功能及IBD进展中的作用。这项研究的目的是确定患病的全层结肠标本，包括对结肠动力功能至关重要的神经肌肉区域，是否含有特定的口腔和肠道病原体。此外，我们比较了白种人（CA）和非裔美国人（AA）结肠微生物群的差异。从克罗恩结肠炎（CC）或溃疡性结肠炎（UC）患者进行择期结肠手术时收集了39份人类全层结肠（患病结肠及相邻健康结肠）标本。我们通过对16S rRNA基因区域进行扩增子测序，从结肠标本中分离并分析细菌核糖体RNA（rRNA）。通过使用微生物生态学定量洞察（QIIME）平台进行分析，将微生物群比例定量为操作分类单元（OTU）。通过QIIME分析鉴定出228种不同的细菌物种。然而，我们只能解读其中53种细菌的物种名称。我们的结果表明，与相邻健康结肠标本相比，CC或UC结肠样本中无害细菌的比例发生了改变。我们进一步首次在全层结肠标本中表明，CC和UC患病标本的微生物群以属于厚壁菌门（）和梭杆菌门（）的假定口腔病原体为主。此外，我们已经确定了CA和AA标本之间微生物群水平的差异模式，这对健康差异研究具有潜在意义。总体而言，我们的结果表明口腔和肠道微生物在调节结肠炎患者结肠动力方面存在显著关联。
