Xu Ye, Zhang Wendiao, Chen Yong, Shan Wenzhe
Nanoscale Science and Technology Laboratory, Institute for Advanced Study, Nanchang University, Nanchang, Jiangxi 330031, PR China.
Department of Civil Engineering, Nanchang University, Nanchang, Jiangxi 330031, PR China.
Data Brief. 2018 Dec 28;22:605-619. doi: 10.1016/j.dib.2018.12.076. eCollection 2019 Feb.
This DIB article provides details about the trajectory identification and data processing algorithms used in the article "Dynamic single-vesicle tracking of cell-bound membrane vesicles on resting, activated, and cytoskeleton-disrupted cells" (Zhang et al.) [1]. The algorithm identifies vesicles on cell membranes from series of undyed grayscale images captured by the confocal microscope based on contrast differences and then trajectories of vesicles are obtained by analyzing their positions in consecutive images. Once the trajectories have been obtained, other quantitative movement information, such as moving speed, direction and acceleration, are derived by standard dynamic relations.
这篇数据说明文章详细介绍了《静息、激活和细胞骨架破坏细胞上结合细胞膜囊泡的动态单囊泡追踪》（Zhang等人）[1]一文中使用的轨迹识别和数据处理算法。该算法基于对比度差异，从共聚焦显微镜拍摄的一系列未染色灰度图像中识别细胞膜上的囊泡，然后通过分析其在连续图像中的位置来获得囊泡的轨迹。一旦获得轨迹，其他定量运动信息，如移动速度、方向和加速度，可通过标准动力学关系得出。
