细菌精子症、宿主及生活方式因素对精子DNA完整性的影响：一项基于尼泊尔一家生育中心的横断面研究。
Influence of Bacteriospermia, Host and Lifestyle Factors on Sperm DNA Integrity: A Cross-Sectional Study Based on a Fertility Center of Nepal.
作者信息
Shrestha Anima, Joshi Dev Raj, Vaidya Dijan, Shrestha Sanu Maiya, Singh Anjana
机构信息
Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal.
Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal.
出版信息
J Family Reprod Health. 2025 Mar;19(1):37-43. doi: 10.18502/jfrh.v19i1.18438.
OBJECTIVE
To determine the sperm DNA fragmentation in the semen of suspected sub-fertile men of Nepal and find its association with bacteriospermia demographic and lifestyle factors.
MATERIALS AND METHODS
A cross-sectional study was conducted with the subjects, males among sub-fertile couples visiting a fertility center in Kathmandu Valley, Nepal for consultation. Information on demography and exposure factors was obtained with a structured questionnaire, and bacteriospermia and sperm DNA fragmentation was determined from the semen samples collected from the study subjects. The data obtained were used to assess sperm DNA fragmentation and its association with various risk factors in sub-fertile men of Nepal.
RESULTS
Out of 186 samples analyzed, 41.4% had low DNA fragmentation (<15%), 38.7% had moderate DNA fragmentation (≥15% and <30%), and 19.9% had high DNA fragmentation (≥30%). Among the risk factors analyzed, sperm DNA fragmentation was found to be significantly associated with the age of the patients (p<0.05). Other factors analyzed body mass index, smoking, alcohol consumption, physical activity, and bacteriospermia were not found to be associated with sperm DNA fragmentation in our study.
CONCLUSION
Sperm DNA integrity may be distorted with the increasing age of men, leading to decreasing fertility potential.
目的
确定尼泊尔疑似亚生育男性精液中的精子DNA片段化情况，并找出其与细菌性精液症、人口统计学和生活方式因素的关联。
材料与方法
对尼泊尔加德满都谷地一家生育中心咨询的亚生育夫妇中的男性进行了一项横断面研究。通过结构化问卷获取人口统计学和暴露因素信息，并从研究对象采集的精液样本中测定细菌性精液症和精子DNA片段化情况。所获数据用于评估尼泊尔亚生育男性的精子DNA片段化及其与各种风险因素的关联。
结果
在分析的186个样本中，41.4%的样本DNA片段化程度低（<15%），38.7%的样本DNA片段化程度中等（≥15%且<30%），19.9%的样本DNA片段化程度高（≥30%）。在分析的风险因素中，发现精子DNA片段化与患者年龄显著相关（p<0.05）。在我们的研究中，分析的其他因素，即体重指数、吸烟、饮酒、身体活动和细菌性精液症，均未发现与精子DNA片段化相关。
结论
男性年龄增长可能会破坏精子DNA完整性，导致生育潜力下降。
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